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Thymic stem cells in mouse bone marrow
M Antica, L Wu, K Shortman and R Scollay
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria,
NSW, Australia.
There is still controversy concerning the nature of the stem cells from
bone marrow that colonize the thymus during embryogenesis and continually
throughout life. To identify the bone marrow stem cells that home to and
populate the thymus, we screened murine bone marrow cells for the presence
of a population of surface phenotype similar to the earliest known
intrathymic precursor. We have identified a population characterized by
expression of an intermediate level of heat stable antigen, a very low
level of Thy-1, and high levels of CD44 and class I major
histocompatibility complex antigens. It is negative for B- cell,
granulocyte, macrophage, and erythrocyte markers (B220, Gr-1, Mac- 1, and
TER 119). All these markers are common to the intrathymic precursors and
bone marrow stem cells. However, this new bone marrow population is Sca-2+,
similar to the intrathymic precursor, which makes a clear distinction from
the Sca-2- bone marrow hematopoietic stem cells previously characterized.
The frequency of the new population in the normal mouse bone marrow is
about 0.25%. When transferred intrathymically or intravenously to lethally
irradiated mice, it has a higher expansion potential (2 x 10(5)) than has
been found for the intrathymic precursors (10(3)), but less than was found
for the Sca-2- multipotent stem cell (10(7)). These transfer studies also
showed that it was pluripotent, in that its precursor activity was not
restricted to the production of T or B lymphocytes. However, it gave a
reduced spleen colony number and smaller colonies (day-12 colony-forming
unit spleen) when compared with multipotent stem cells. Thus, the cell we
have identified appears to be the latest pluripotent cells so far
identified in bone marrow and is therefore a good candidate for a bone
marrow prothymocyte, but it appears not to be T-cell-committed.
Volume 84,
Issue 1,
pp. 111-117,
07/01/1994
Copyright © 1994 by The American Society of Hematology
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