Generation of T cells from cytokine-mobilized peripheral blood and adult
bone marrow CD34+ cells
AH Galy, S Webb, D Cen, LJ Murray, J Condino, RS Negrin and BP Chen
Experimental Cellular Therapy, Systemix, Inc, Palo Alto 94304.
The present study compared the T-cell progenitor content of CD34+ lineage
(Lin)- cells isolated from normal adult bone marrow (ABM) and mobilized
peripheral blood (MPB). Both cell populations were found to differentiate
into T cells when injected into human fetal thymi implanted into severe
combined immunodeficient mice. Cytokine-MPB cells were less efficient than
ABM cells in engrafting in the fetal human thymus, although both gave rise
to thymocytes with identical phenotypes based on the analysis of CD1a, CD3,
CD4, and CD8 expression. Thymocytes derived from adult CD34+ Lin- cells
were capable of fully differentiating into mature CD3+ T cells expressing
either the T-cell receptor (TCR) gamma delta or the TCR alpha beta (the
later associated with CD4 or CD8), showing that the T-cell progenies of
adult CD34+ cells were polyclonal and functional. Our data indicate that
human MPB CD34+ cells are qualitatively identical to their BM counterparts,
and demonstrate the existence of T-lymphoid progenitor cell activity in
MPB.
Volume 84,
Issue 1,
pp. 104-110,
07/01/1994
Copyright © 1994 by The American Society of Hematology
