|
|
 Previous Article | Table of Contents | Next Article 
T lymphocytes in skin lesions of psoriasis and mycosis fungoides express
B7-1: a ligand for CD28
BJ Nickoloff, FO Nestle, XG Zheng and LA Turka
Department of Pathology, University of Michigan Medical School, Ann Arbor.
The activation of T cells requires two distinct signals. One signal
involves interaction of the antigen-specific T-cell receptor with major
histocompatibility complex molecules plus antigenic peptide; a second
signal, which is antigen nonspecific, is the interaction of CD28 with its
natural ligands B7-1 and B7-2/B70. CD28 is expressed on 80% of T cells, is
upregulated after activation, and binds to B7 gene-family members, found on
antigen-presenting cells. Because of our interest in the immunologic basis
of benign and malignant T-cell-mediated disorders of the skin, we
investigated the cellular distribution of CD28 and B7 family members in
lesions of psoriasis and mycosis fungoides. By immunostaining cryostat
sections of skin, CD28 was found to be expressed on virtually all
lymphocytes in the epidermis and dermis of both skin diseases.
Surprisingly, B7-1 was also found to be expressed on virtually all
lymphocytes in the epidermis and dermis of both skin diseases. B7-1
expression was confirmed on CD3+ T lymphocytes using flow cytometry of
single cell suspensions of fresh, unfixed psoriatic lesional tissue. To
exclude the possibility that this result was caused by a second reagent
contaminating the monoclonal antibody (MoAb) preparation, two different
lots were used, and the MoAb was absorbed onto Chinese hamster ovary (CHO)
transfectants expressing B7-1, or vector-only transfected CHO cells. These
procedures confirmed that a B7- 1-like epitope was being recognized on
psoriatic lesional T cells. In contrast to B7-1 expression on lymphocytes,
B7-3, as defined by anti-BB- 1 MoAb reactivity, was found primarily on
epidermal keratinocytes in both skin diseases and was not found on T cells.
These results indicate that within two common skin disorders, lesional T
cells accumulate in the dermis and epidermis, which express B7-1. Such
expression may permit self-costimulation involving the CD28-mediated
activation pathway, and thereby contribute to the ongoing T-cell
proliferation present in these chronic, benign, and malignant skin
diseases.
Volume 83,
Issue 9,
pp. 2580-2586,
05/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. R. Podojil and S. D. Miller
Cross-Linking of CD80 on CD4+ T Cells Activates a Calcium-Dependent Signaling Pathway
J. Immunol.,
January 15, 2009;
182(2):
766 - 773.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Burg, R. Dummer, A. Haeffner, W. Kempf, and M. Kadin
From Inflammation to Neoplasia: Mycosis Fungoides Evolves From Reactive Inflammatory Conditions (Lymphoid Infiltrates) Transforming Into Neoplastic Plaques and Tumors
Arch Dermatol,
July 1, 2001;
137(7):
949 - 952.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Abrams, S. L. Kelley, E. Hayes, T. Kikuchi, M. J. Brown, S. Kang, M. G. Lebwohl, C. A. Guzzo, B. V. Jegasothy, P. S. Linsley, et al.
Blockade of T Lymphocyte Costimulation with Cytotoxic T Lymphocyte-associated Antigen 4-Immunoglobulin (CTLA4Ig) Reverses the Cellular Pathology of Psoriatic Plaques, Including the Activation of Keratinocytes, Dendritic Cells, and Endothelial Cells
J. Exp. Med.,
September 5, 2000;
192(5):
681 - 694.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
