|
|
 Previous Article | Table of Contents | Next Article 
Monocyte tissue factor induction by lipopolysaccharide (LPS): dependence on
LPS-binding protein and CD14, and inhibition by a recombinant fragment of
bactericidal/permeability-increasing protein
K Meszaros, S Aberle, R Dedrick, R Machovich, A Horwitz, C Birr, G Theofan and JB Parent
XOMA Corp, Berkeley, CA 94710.
Mononuclear phagocytes, stimulated by bacterial lipopolysaccharide (LPS),
have been implicated in the activation of coagulation in sepsis and
endotoxemia. In monocytes LPS induces the synthesis of tissue factor (TF)
which, assembled with factor VII, initiates the blood coagulation cascades.
In this study we investigated the mechanism of LPS recognition by
monocytes, and the consequent expression of TF mRNA and TF activity. We
also studied the inhibition of these effects of LPS by rBPI23, a 23-kD
recombinant fragment of bactericidal/permeability increasing protein, which
has been shown to antagonize LPS in vitro and in vivo. Human peripheral
blood mononuclear cells, or monocytes isolated by adherence, were
stimulated with Escherichia coli O113 LPS at physiologically relevant
concentrations (> or = 10 pg/mL). The effect of LPS was dependent on the
presence of the serum protein LBP (lipopolysaccharide-binding protein), as
shown by the potentiating effect of human recombinant LBP or serum.
Furthermore, recognition of low amounts of LPS by monocytes was also
dependent on CD14 receptors, because monoclonal antibodies against CD14
greatly reduced the LPS sensitivity of monocytes in the presence of serum
or rLBP. Induction of TF activity and mRNA expression by LPS were inhibited
by rBPI23. The expression of tumor necrosis factor showed qualitatively
similar changes. Considering the involvement of LPS-induced TF in the
potentially lethal intravascular coagulation in sepsis, inhibition of TF
induction by rBPI23 may be of therapeutic benefit.
Volume 83,
Issue 9,
pp. 2516-2525,
05/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. W. McMichael, A. Roghanian, L. Jiang, R. Ramage, and J.-M. Sallenave
The Antimicrobial Antiproteinase Elafin Binds to Lipopolysaccharide and Modulates Macrophage Responses
Am. J. Respir. Cell Mol. Biol.,
May 1, 2005;
32(5):
443 - 452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Mattsson, T. Hartung, S. Morath, and A. Egesten
Highly Purified Lipoteichoic Acid from Staphylococcus aureus Induces Procoagulant Activity and Tissue Factor Expression in Human Monocytes but Is a Weak Inducer in Whole Blood: Comparison with Peptidoglycan
Infect. Immun.,
July 1, 2004;
72(7):
4322 - 4326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-Z. Cui, G. Zhao, A. L. Winokur, E. Laag, J. R. Bydash, M. S. Penn, G. M. Chisolm, and X. Xu
Lysophosphatidic Acid Induction of Tissue Factor Expression in Aortic Smooth Muscle Cells
Arterioscler. Thromb. Vasc. Biol.,
February 1, 2003;
23(2):
224 - 230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Aga, C. J. Johnson, A. P. Hart, A. G. Guadarrama, M. Suresh, J. Svaren, P. J. Bertics, and B. J. Darien
Modulation of monocyte signaling and pore formation in response to agonists of the nucleotide receptor P2X7
J. Leukoc. Biol.,
July 1, 2002;
72(1):
222 - 232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Mattsson, H. Herwald, L. Bjorck, and A. Egesten
Peptidoglycan from Staphylococcus aureus Induces Tissue Factor Expression and Procoagulant Activity in Human Monocytes
Infect. Immun.,
June 1, 2002;
70(6):
3033 - 3039.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Nube and M. P. C. Grooteman
Impact of contaminated dialysate on long-term haemodialysis-related complications: is it really that important?
Nephrol. Dial. Transplant.,
October 1, 2001;
16(10):
1986 - 1991.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ferro, S. Parrotto, S. Basili, C. Alessandri, and F. Violi
Simvastatin inhibits the monocyte expression of proinflammatory cytokines in patients with hypercholesterolemia
J. Am. Coll. Cardiol.,
August 1, 2000;
36(2):
427 - 431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kjalke, A. Silveira, A. Hamsten, U. Hedner, and M. Ezban
Plasma Lipoproteins Enhance Tissue Factor-Independent Factor VII Activation
Arterioscler. Thromb. Vasc. Biol.,
July 1, 2000;
20(7):
1835 - 1841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ferro, S. Basili, D. Pratico, L. Iuliano, G. A. FitzGerald, and F. Violi
Vitamin E Reduces Monocyte Tissue Factor Expression in Cirrhotic Patients
Blood,
May 1, 1999;
93(9):
2945 - 2950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Saliola, R Lorenzet, D Ferro, S Basili, C Caroselli, A Di Santo, M Sallese, and F Violi
Enhanced expression of monocyte tissue factor in patients with liver cirrhosis
Gut,
September 1, 1998;
43(3):
428 - 432.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Abrahamson, H.-M. Wu, R. E. Williams, K. Der, N. Ottah, R. Little, H. Gazzano-Santoro, G. Theofan, R. Bauer, S. Leigh, et al.
Biochemical Characterization of Recombinant Fusions of Lipopolysaccharide Binding Protein and Bactericidal/ Permeability-increasing Protein. IMPLICATIONS IN BIOLOGICAL ACTIVITY
J. Biol. Chem.,
January 24, 1997;
272(4):
2149 - 2155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Steve Ammons and C. Mallari
An N-terminal fragment of bactericidal/permeability-increasing protein protects against hemodynamic and metabolic derangements in rat Gram-negative sepsis
Innate Immunity,
February 1, 1996;
3(1):
57 - 66.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Sanlioglu, C. M. Williams, L. Samavati, N. S. Butler, G. Wang, P. B. McCray Jr., T. C. Ritchie, G. W. Hunninghake, E. Zandi, and J. F. Engelhardt
Lipopolysaccharide Induces Rac1-dependent Reactive Oxygen Species Formation and Coordinates Tumor Necrosis Factor-alpha Secretion through IKK Regulation of NF-kappa B
J. Biol. Chem.,
August 3, 2001;
276(32):
30188 - 30198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
