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Analysis of interphase cells for the Philadelphia translocation using
painting probe made by inter-Alu-polymerase chain reaction from a radiation
hybrid
DC Seong, MY Song, EP Henske, SO Zimmerman, RE Champlin, AB Deisseroth and MJ Siciliano
Department of Hematology, University of Texas M.D. Anderson Cancer Center,
Houston 77030.
Fluorescence in situ hybridization (FISH) probe for the identification of
the Philadelphia (Ph) translocation [t(9;22) (q34;q11)] in chronic
myelogenous leukemia cells was developed by inter-Alu-polymerase chain
reaction of DNA from an interspecific somatic cell hybrid containing
approximately 5 Mb of human DNA covering the ABL gene region on human
chromosome 9q34. This probe was large enough to be effective in identifying
the genomic domains yet small enough to resolve them in more than 90% of
bone marrow interphase cells. Combination of the probe with a cosmid contig
probe for the BCR region of chromosome 22 in two- color FISH reduced the
frequency of false-positive identification of the Ph chromosome to less
than 1%. The procedure allows detection of as few as 1% Ph+ cells
independent of the cycling status or BCR/ABL expression level of cells, and
the quantitation of non-Ph chromosome- containing interphase nuclei in the
marrow of patients judged 100% Ph+ by standard cytogenetics.
Volume 83,
Issue 8,
pp. 2268-2273,
04/15/1994
Copyright © 1994 by The American Society of Hematology
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