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Bcl2 inhibits apoptosis associated with terminal differentiation of HL- 60
myeloid leukemia cells
L Naumovski and ML Cleary
Department of Pediatrics, Lucile Salter Packard Children's Hospital at
Stanford, CA.
The Bcl2 protein inhibits apoptosis (programmed cell death) induced by a
variety of noxious stimuli. However, relatively little is known about its
effect on apoptosis that occurs after terminal differentiation. Bcl2
protein levels decrease during differentiation of myeloid cells into
granulocytes that subsequently undergo apoptosis, but the potential role of
Bcl2 in coupling survival and differentiation remains undefined. To
ascertain the relationship between decreasing Bcl2 levels and the onset of
apoptosis in differentiating myeloid cells, Bcl2 was hyperexpressed in the
HL-60 cell line after retroviral gene transfer. After treatment of
HL-60/BCL2 cells with all-trans retinoic acid or phorbol myristic acid,
Bcl2 levels did not decrease as in normal HL-60 cells but, rather,
increased because of activation of the viral promoter. Differentiation of
the Bcl2-overexpressing cells was similar to that of normal HL-60 cells,
but they showed little evidence for apoptosis and had a prolonged survival.
These studies show that the survival-enhancing properties of Bcl2
counteract programmed cell death that accompanies terminal differentiation;
however, Bcl2 has no significant effect on differentiation itself,
suggesting that apoptosis and differentiation are regulated independently
in myeloid cells.
Volume 83,
Issue 8,
pp. 2261-2267,
04/15/1994
Copyright © 1994 by The American Society of Hematology
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|
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|
|
|
|
|
