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Expansion of T cells expressing low CD4 or CD8 levels in B-cell chronic
lymphocytic leukemia: correlation with disease status and neoplastic
phenotype
U Dianzani, P Omede, F Marmont, D DiFranco, A Fusaro, M Bragardo, V Redoglia, F Giaretta, L Mairone and M Boccadoro
Dipartimento di Scienze Mediche, Universita di Torino, Novara, Italy.
Peripheral blood (PB) T cells from 56 patients with B-cell chronic
lymphocytic leukemia (B-CLL) were analyzed by two- and three-color
immunofluorescence (IF) to determine the expansion of distinct T-cell
subsets and their relationship with the clinical and biological features of
the disease. We detected the expansion of an unusual T-cell subpopulation
expressing lower CD4 or CD8 levels (CD4lo, CD8lo) than classic T cells
(CD4hi, CD8hi). This subpopulation also expressed low levels of the CD3/TCR
alpha/beta complex and was CD19-CD13-CD14-. A phenotypic analysis probing
the activation level of CD4lo, CD8lo, CD4hi, and CD8hi cells showed that
they comprised increased counts of HLA-DR+, CD11b+, CD45R0+, and CD45RA+
cells. Subset expansion ranged from 2.1- to 13.6-fold. Statistical analysis
showed that the size of some of these subsets was correlated to intrinsic
features of the tumor. First, CD4loHLA-DR+ cell counts were higher in
patients with stage A than those with stages B and C disease. Second,
CD8loHLA-DR+ cell counts were higher in patients in stable remission than
in those at diagnosis. Third, CD4loHLA-DR+, CD4loCD45R0+, CD4loCD45RA+, and
CD4hiCD11b+ cell counts were higher in patients whose tumor cells expressed
high levels of surface immunoglobulin (sIg) than in those expressing low
levels. The involvement of CD4lo and CD8lo cells in most of these
correlations suggests that they may be tumor-reactive cells. Similar cells
described in human and murine autoimmune disease have been shown to be
autoreactive anergic cells, which may derive from nonclassic pathways of
T-cell development.
Volume 83,
Issue 8,
pp. 2198-2205,
04/15/1994
Copyright © 1994 by The American Society of Hematology
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