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Expression of type II activin receptor genes during differentiation of
human K562 cells and cDNA cloning of the human type IIB activin receptor
K Hilden, T Tuuri, M Eramaa and O Ritvos
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Recent studies have indicated that activin A/erythroid differentiation
factor is a physiologic hematopoietic growth and differentiation factor
mainly for cells of the erythroid lineage. We studied the expression of the
two type II activin receptor mRNAs during the differentiation of K562
erythroleukemic cells, which are known to be induced toward the erythroid
lineage in response to activin or toward the megakaryoblastic lineage by
phorbol myristate acetate (PMA). The cDNA of the human activin receptor
type IIB (hActR-IIB) was cloned and sequenced from two RNA sources, the
K562 cells and the human fetal brain, which is, of the tissues screened by
Northern blot analysis, the most abundant source of ActR-IIB RNA. The cDNA
encodes a predicted 512 amino acid protein containing an extracellular
ligand binding domain, a hydrophobic transmembrane domain, and an
intracellular serine/threonine kinase domain. The amino acid sequence is
99.2% and 98.4% homologous in the coding region to the previously described
mouse and rat ActR-IIB2s, respectively, and 69% identical to the other
human activin serine/threonine kinase receptor, hActR-II. The alternative
splicing events in the juxtamembrane region previously reported for the
respective mouse receptor were not observed during the processing of K562
cell and human fetal brain RNA. Northern analysis showed that the 10- and
2.5-kb transcripts of hActR-IIB are more abundantly expressed than the 6.0-
and 3.0-kb transcripts of hActR-II in K562 cells. No changes in the
steady-state levels of hActR-II and IIB mRNAs were detected upon
differentiation of K562 cells by activin A or by PMA. Similarly, the
receptor mRNA levels remained constant in HL-60 cells induced to either
monocyte/macrophage or granulocyte-like cells by PMA or dimethyl sulfoxide,
respectively. Thus, the mRNA expression levels of both receptors apparently
do not correlate with the differentiation status of these cells.
Volume 83,
Issue 8,
pp. 2163-2170,
04/15/1994
Copyright © 1994 by The American Society of Hematology
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