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c-kit expression in human megakaryoblastic leukemia cell lines
ZB Hu, W Ma, CC Uphoff, H Quentmeier and HG Drexler
DSM-German Collection of Microorganisms and Cell Cultures, Department of
Human and Animal Cell Cultures, Braunschweig.
A panel of 164 continuous human leukemia-lymphoma cell lines was analyzed
for expression of c-kit using Northern blotting and reverse
transcriptase-polymerase chain reaction (RT-PCR). The c-kit transcripts
were detectable in cell lines assigned to the myeloid (in 7 of 29 by
Northern blotting and in 4 of 8 by RT-PCR), monocytic (in 1 of 24 by
Northern blotting and in 3 of 6 by RT-PCR), erythroid (in 6 of 8 by
Northern blotting and in 5 of 5 by RT-PCR), and megakaryoblastic (in 10 of
10 by Northern blotting) lineages, c-kit expression was not seen by
Northern blotting or RT-PCR analysis in any of the 93 lymphoid leukemia,
myeloma, or lymphoma cell lines. Treatment of four megakaryoblastic cell
lines with protein kinase C activators (phorbol ester
12-O-tetradecanoylphorbol 13-acetate and Bryostatin 1) led to terminal
differentiation as assessed by morphologic alterations, changes in the
surface marker profile, and growth arrest. These effects were associated
with enhanced c-kit mRNA expression. Exposure to all- trans retinoic acid
down-regulated c-kit mRNA levels, while simultaneously causing morphologic
alterations in all four cell lines. Stimulation with growth factors
(interleukin-3, granulocyte macrophage- colony stimulating factor, and
insulin-like growth factors I and II), used to assess any role of c-kit in
proliferative processes, did not lead to significant upregulation or
downregulation of c-kit expression. The finding of constitutive and high
expression of c-kit mRNA in all megakaryoblastic leukemia cell lines and
its modulation by various reagents might further contribute to the
understanding of megakaryopoietic proliferation, differentiation, and
leukemogenesis.
Volume 83,
Issue 8,
pp. 2133-2144,
04/15/1994
Copyright © 1994 by The American Society of Hematology
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