|
|
 Previous Article | Table of Contents | Next Article 
Modulation of megakaryocytopoiesis by human basic fibroblast growth factor
H Avraham, N Banu, DT Scadden, J Abraham and JE Groopman
Division of Hematology/Oncology, New England Deaconess Hospital, Boston, MA
02215.
Basic fibroblast growth factor (bFGF) may act to modulate hematopoiesis in
addition to its effects on mesenchymal cells. We studied the effects of
bFGF on human and murine primary marrow megakaryocytes. bFGF modestly
enhanced the size of the human megakaryocyte colony-forming unit (CFU-MK)
and cell numbers per colony, in combination with interleukin-3 (IL-3) or
granulocyte-macrophage colony stimulating factor (GM-CSF). Adhesion of
human megakaryocytes to bone marrow (BM) stromal fibroblasts was enhanced
when either stromal fibroblasts or megakaryocytes were treated with bFGF.
This resulted in significantly increased proliferation of megakaryocytes.
In addition, bFGF augmented secretion of the cytokines tumor necrosis
factor alpha and IL-6 by human primary BM megakaryocytes. Immature murine
megakaryocytes showed a significant growth response to bFGF as measured by
the single cell growth assay. This effect was abrogated by specific
antibodies for bFGF and combination of anti-IL-6 and anti-IL-1 beta
antibodies. bFGF has no effect on murine CFU-MK formation, but
significantly potentiated CFU-MK formation in the presence of IL-3 or
GM-CSF. These results indicate that the effect of bFGF on various
megakaryocyte populations is different and that bFGF may affect
megakaryocytopoiesis via modulation of megakaryocyte-stromal interactions
and via augmentation of cytokine secretion from megakaryocytes.
Volume 83,
Issue 8,
pp. 2126-2132,
04/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Nakayama, N. Mutsuga, and G. Tosato
FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc
Blood,
February 15, 2007;
109(4):
1363 - 1372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-G. Kopp, S. T. Avecilla, A. T. Hooper, and S. Rafii
The Bone Marrow Vascular Niche: Home of HSC Differentiation and Mobilization
Physiology,
October 1, 2005;
20(5):
349 - 356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Banu, S. Avraham, and H. K. Avraham
P-Selectin, and Not E-Selectin, Negatively Regulates Murine Megakaryocytopoiesis
J. Immunol.,
October 15, 2002;
169(8):
4579 - 4585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Deng, N. Banu, B. Malloy, P. Hass, J. F. Wang, L. Cavacini, D. Eaton, and H. Avraham
An Agonist Murine Monoclonal Antibody to the Human c-Mpl Receptor Stimulates Megakaryocytopoiesis
Blood,
September 15, 1998;
92(6):
1981 - 1988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ortega, M. Ittmann, S. H. Tsang, M. Ehrlich, and C. Basilico
Neuronal defects and delayed wound healing in mice lacking fibroblast growth factor 2
PNAS,
May 12, 1998;
95(10):
5672 - 5677.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Yuen, L. Mittal, C.-X. Deng, and K. Choi
Generation of a Primitive Erythroid Cell Line and Promotion of Its Growth by Basic Fibroblast Growth Factor
Blood,
May 1, 1998;
91(9):
3202 - 3209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bikfalvi, S. Klein, G. Pintucci, and D. B. Rifkin
Biological Roles of Fibroblast Growth Factor-2
Endocr. Rev.,
February 1, 1997;
18(1):
26 - 45.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. Mohle, D. Green, M. A. S. Moore, R. L. Nachman, and S. Rafii
Constitutive production and thrombin-induced release of vascular endothelial growth factor by human megakaryocytes and platelets
PNAS,
January 21, 1997;
94(2):
663 - 668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
