|
|
 Previous Article | Table of Contents | Next Article 
Platelets inhibit the induction of nitric oxide synthesis by interleukin-1
beta in vascular smooth muscle cells
W Durante, VB Schini, MH Kroll, S Catovsky, T Scott-Burden, JG White, PM Vanhoutte and AI Schafer
Medical Service, Houston Veterans Affairs Medical Center, TX 77030.
We have investigated the role of platelets in regulating the hemostatic and
vasomotor properties of vascular smooth muscle. Experiments were performed
to examine the effect of the releasate from activated platelets on the
production of nitric oxide from interleukin-1 beta (IL- 1 beta)-treated
cultured rat aortic smooth muscle cells. Treatment of vascular smooth
muscle cells with IL-1 beta resulted in significant accumulation of nitrite
in the culture media and in marked elevation of intracellular cyclic
guanosine monophosphate (GMP) levels. The releasate from
collagen-aggregated platelets blocked the IL-1 beta- mediated production of
nitrite and the accumulation of cyclic GMP in smooth muscle cells in a
platelet number-dependent manner. In functional assays, the perfusates from
columns containing IL-1 beta- treated smooth muscle cells relaxed detector
blood vessels without endothelium and the addition of IL-1 beta-treated
smooth muscle cells to suspensions of platelets inhibited their
thrombin-induced aggregation. The simultaneous treatment of smooth muscle
cells with IL- 1 beta and the platelet releasate abolished both the
vasorelaxing activities of the perfusates and the inhibition of platelet
aggregation. Platelet releasates treated with a neutralizing antibody to
platelet-derived growth factor (PDGF) failed to block IL-1 beta- induced
nitric oxide production by the smooth muscle cells, as measured by both
biochemical and functional assays. The platelet releasate from a patient
with gray platelet syndrome likewise failed to block IL-1 beta-induced
nitrite release by smooth muscle cells. These results demonstrate that
platelets downregulate the production of nitric oxide by IL-1 beta-treated
vascular smooth muscle cells through the release of PDGF. This effect may
represent a novel mechanism by which platelets regulate vasomotor tone and
thrombus formation at sites of vascular injury.
Volume 83,
Issue 7,
pp. 1831-1838,
04/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Stuart-Smith and J. Y. Jeremy
Microvessel damage in acute respiratory distress syndrome: the answer may not be NO
Br. J. Anaesth.,
August 1, 2001;
87(2):
272 - 279.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Durante, K. J. Peyton, and A. I. Schafer
Platelet-Derived Growth Factor Stimulates Heme Oxygenase-1 Gene Expression and Carbon Monoxide Production in Vascular Smooth Muscle Cells
Arterioscler. Thromb. Vasc. Biol.,
November 1, 1999;
19(11):
2666 - 2672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yamawaki, K. Sato, M. Hori, H. Ozaki, S.-I. Nakamura, H. Nakayama, K. Doi, and H. Karaki
Impairment of EDR by a long-term PDGF treatment in organ-cultured rabbit mesenteric artery
Am J Physiol Heart Circ Physiol,
July 1, 1999;
277(1):
H318 - H323.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Gonzalez-Fernandez, A. Lopez-Farre, J. A. Rodriguez-Feo, J. Farre, J. Guerra, J. Fortes, I. Millas, M. Garcia-Duran, L. Rico, P. Mata, et al.
Expression of Inducible Nitric Oxide Synthase After Endothelial Denudation of the Rat Carotid Artery : Role of Platelets
Circ. Res.,
November 30, 1998;
83(11):
1080 - 1087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Loppnow, R. Bil, S. Hirt, U. Schonbeck, M. Herzberg, K. Werdan, E. Theodor Rietschel, E. Brandt, and H.-D. Flad
Platelet-Derived Interleukin-1 Induces Cytokine Production, but not Proliferation of Human Vascular Smooth Muscle Cells
Blood,
January 1, 1998;
91(1):
134 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ikeda, C. Morita, M. Mizuno, T. Sada, H. Koike, and K. Kurokawa
PDGF-BB decreases systolic blood pressure through an increase in macrovascular compliance in rats
Am J Physiol Heart Circ Physiol,
October 1, 1997;
273(4):
H1719 - H1726.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Durante, M. H. Kroll, N. Christodoulides, K. J. Peyton, and A. I. Schafer
Nitric Oxide Induces Heme Oxygenase-1 Gene Expression and Carbon Monoxide Production in Vascular Smooth Muscle Cells
Circ. Res.,
April 19, 1997;
80(4):
557 - 564.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. L. Farre, J. R. Mosquera, L. S. de Miguel, I. Millas, T. de Frutos, M. Monton, M. P. Sierra, A. Riesco, and S. Casado
Endothelial Cells Inhibit NO Generation by Vascular Smooth Muscle Cells: Role of Transforming Growth Factor-ß
Arterioscler. Thromb. Vasc. Biol.,
October 1, 1996;
16(10):
1263 - 1268.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Durante, L. Liao, I. Iftikhar, K. Cheng, and A. I. Schafer
Platelet-derived Growth Factor Regulates Vascular Smooth Muscle Cell Proliferation by Inducing Cationic Amino Acid Transporter Gene Expression
J. Biol. Chem.,
May 17, 1996;
271(20):
11838 - 11843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
