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Establishment of two permanent human bone marrow stromal cell lines with
long-term post irradiation feeder capacity
K Thalmeier, P Meissner, G Reisbach, M Falk, A Brechtel and P Dormer
Institut fur Experimentelle Hamatologie, GSF-Forschungszentrum fur Unwelt
und Gesundheit, GmbH, Munchen, Germany.
We describe the establishment of two permanent Simian virus 40- transformed
human stromal cell lines, designated L87/4 and L88/5, derived from the bone
marrow of a hematologically normal male patient. Both cell lines show a
fibroblastoid morphology and do not express hematopoietic cell markers.
L87/4 but not L88/5 expresses the macrophage marker CD68. The most
remarkable feature of these new stromal cell lines is their ability to
persist as growth-arrested adherent feeder cells after ionizing-irradiation
at doses up to, and exceeding 20 Gy (L87/4). This renders them particularly
useful for studying aspects of feeder dependence of hematopoietic cell
development in long-term culture. Both cell lines are able to function as
feeder cells, supporting the long-term proliferation of CD34+ human cord
blood cells as well as the clonogenic growth of the human Burkitt lymphoma
B- cell line BL70.
Volume 83,
Issue 7,
pp. 1799-1807,
04/01/1994
Copyright © 1994 by The American Society of Hematology
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