|
|
 Previous Article | Table of Contents | Next Article 
Isolation and characterization of gelatinase granules from human
neutrophils
L Kjeldsen, H Sengelov, K Lollike, MH Nielsen and N Borregaard
Department of Hematology, State University Hospital, Rigshospitalet,
Kobenhavn, Denmark.
We recently confirmed the existence of gelatinase granules as a
subpopulation of peroxidase-negative granules by double-labeling immunogold
electron microscopy on intact cells and by subcellular fractionation.
Further characterization of gelatinase granules has been hampered by poor
separation of specific and gelatinase granules on both two-layer Percoll
gradients and sucrose gradients. We have developed a three-layer Percoll
density gradient that allows separation of the different granules and
vesicles from human neutrophils; in particular, it allows separation of
specific and gelatinase granules. This allows us to characterize these two
granule populations with regard to their content of membrane proteins,
which become incorporated into the plasma membrane during exocytosis. We
found that gelatinase granules, defined as peroxidase-negative granules
containing gelatinase but lacking lactoferrin, contain 50% of total cell
gelatinase, with the remaining residing in specific granules. Furthermore,
we found that 20% to 25% of both the adhesion protein Mac-1 and the
NADPH-oxidase component cytochrome b558 is localized in gelatinase
granules. Although no qualitative difference was observed between specific
granules and gelatinase granules with respect to cytochrome b558 and Mac-1,
stimulation of the neutrophil with FMLP resulted in a selective
mobilization of the least dense peroxidase-negative granules, ie,
gelatinase granules, which, in concert with secretory vesicles, furnish the
plasma membrane with Mac-1 and cytochrome b558. This shows that gelatinase
granules are functionally important relative to specific granules in
mediating early inflammatory responses.
Volume 83,
Issue 6,
pp. 1640-1649,
03/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. M. Gibbs, K. Roelants, and M. K. O'Bryan
The CAP Superfamily: Cysteine-Rich Secretory Proteins, Antigen 5, and Pathogenesis-Related 1 Proteins--Roles in Reproduction, Cancer, and Immune Defense
Endocr. Rev.,
December 1, 2008;
29(7):
865 - 897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Uriarte, D. W. Powell, G. C. Luerman, M. L. Merchant, T. D. Cummins, N. R. Jog, R. A. Ward, and K. R. McLeish
Comparison of Proteins Expressed on Secretory Vesicle Membranes and Plasma Membranes of Human Neutrophils
J. Immunol.,
April 15, 2008;
180(8):
5575 - 5581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Busetto, E. Trevisan, E. Decleva, P. Dri, and R. Menegazzi
Chloride Movements in Human Neutrophils during Phagocytosis: Characterization and Relationship to Granule Release
J. Immunol.,
September 15, 2007;
179(6):
4110 - 4124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Kasper, J. D. Glaeser, S. Geissler, A. Ode, J. Tuischer, G. Matziolis, C. Perka, and G. N. Duda
Matrix Metalloprotease Activity Is an Essential Link Between Mechanical Stimulus and Mesenchymal Stem Cell Behavior
Stem Cells,
August 1, 2007;
25(8):
1985 - 1994.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. R. Jog, M. J. Rane, G. Lominadze, G. C. Luerman, R. A. Ward, and K. R. McLeish
The actin cytoskeleton regulates exocytosis of all neutrophil granule subsets
Am J Physiol Cell Physiol,
May 1, 2007;
292(5):
C1690 - C1700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Bedard and K.-H. Krause
The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Physiol Rev,
January 1, 2007;
87(1):
245 - 313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pellme, M. Morgelin, H. Tapper, U.-H. Mellqvist, C. Dahlgren, and A. Karlsson
Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles
J. Leukoc. Biol.,
March 1, 2006;
79(3):
564 - 573.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Lominadze, D. W. Powell, G. C. Luerman, A. J. Link, R. A. Ward, and K. R. McLeish
Proteomic Analysis of Human Neutrophil Granules
Mol. Cell. Proteomics,
October 1, 2005;
4(10):
1503 - 1521.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Faurschou, S. Kamp, J. B. Cowland, L. Udby, A. H. Johnsen, J. Calafat, H. Winther, and N. Borregaard
Prodefensins are matrix proteins of specific granules in human neutrophils
J. Leukoc. Biol.,
September 1, 2005;
78(3):
785 - 793.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Lominadze, M. J. Rane, M. Merchant, J. Cai, R. A. Ward, and K. R. McLeish
Myeloid-Related Protein-14 Is a p38 MAPK Substrate in Human Neutrophils
J. Immunol.,
June 1, 2005;
174(11):
7257 - 7267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Borregaard, K. Theilgaard-Monch, J. B. Cowland, M. Stahle, and O. E. Sorensen
Neutrophils and keratinocytes in innate immunity--cooperative actions to provide antimicrobial defense at the right time and place
J. Leukoc. Biol.,
April 1, 2005;
77(4):
439 - 443.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. IJdo and A. C. Mueller
Neutrophil NADPH Oxidase Is Reduced at the Anaplasma phagocytophilum Phagosome
Infect. Immun.,
September 1, 2004;
72(9):
5392 - 5401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Galant, M. Berliere, D. Dubois, J.-C. Verougstraete, A. Charles, P. Lemoine, I. Kokorine, Y. Eeckhout, P. J. Courtoy, and E. Marbaix
Focal Expression and Final Activity of Matrix Metalloproteinases May Explain Irregular Dysfunctional Endometrial Bleeding
Am. J. Pathol.,
July 1, 2004;
165(1):
83 - 94.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Levesque, F. Liu, P. J. Simmons, T. Betsuyaku, R. M. Senior, C. Pham, and D. C. Link
Characterization of hematopoietic progenitor mobilization in protease-deficient mice
Blood,
July 1, 2004;
104(1):
65 - 72.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Mansfield, S. S. Carey, V. Hinkovska-Galcheva, J. A. Shayman, and L. A. Boxer
Ceramide inhibition of phospholipase D and its relationship to RhoA and ARF1 translocation in GTP{gamma}S-stimulated polymorphonuclear leukocytes
Blood,
March 15, 2004;
103(6):
2363 - 2368.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Dziarski, K. A. Platt, E. Gelius, H. Steiner, and D. Gupta
Defect in neutrophil killing and increased susceptibility to infection with nonpathogenic gram-positive bacteria in peptidoglycan recognition protein-S (PGRP-S)-deficient mice
Blood,
July 15, 2003;
102(2):
689 - 697.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhou, S. A. Stohlman, D. R. Hinton, and N. W. Marten
Neutrophils Promote Mononuclear Cell Infiltration During Viral-Induced Encephalitis
J. Immunol.,
March 15, 2003;
170(6):
3331 - 3336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Udby, J. Calafat, O. E. Sorensen, N. Borregaard, and L. Kjeldsen
Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative granules of neutrophils and in the granules of eosinophils
J. Leukoc. Biol.,
September 1, 2002;
72(3):
462 - 469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. D. Cataldo, K. G. Tournoy, K. Vermaelen, C. Munaut, J.-M. Foidart, R. Louis, A. Noel, and R. A. Pauwels
Matrix Metalloproteinase-9 Deficiency Impairs Cellular Infiltration and Bronchial Hyperresponsiveness during Allergen-Induced Airway Inflammation
Am. J. Pathol.,
August 1, 2002;
161(2):
491 - 498.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhou, S. A. Stohlman, R. Atkinson, D. R. Hinton, and N. W. Marten
Matrix Metalloproteinase Expression Correlates with Virulence following Neurotropic Mouse Hepatitis Virus Infection
J. Virol.,
June 27, 2002;
76(15):
7374 - 7384.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bylund, A. Karlsson, F. Boulay, and C. Dahlgren
Lipopolysaccharide-Induced Granule Mobilization and Priming of the Neutrophil Response to Helicobacter pylori Peptide Hp(2-20), Which Activates Formyl Peptide Receptor-Like 1
Infect. Immun.,
June 1, 2002;
70(6):
2908 - 2914.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Almkvist, C. Dahlgren, H. Leffler, and A. Karlsson
Activation of the Neutrophil Nicotinamide Adenine Dinucleotide Phosphate Oxidase by Galectin-1
J. Immunol.,
April 15, 2002;
168(8):
4034 - 4041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C Cuello, D Wakefield, and N Di Girolamo
Neutrophil accumulation correlates with type IV collagenase/gelatinase activity in endotoxin induced uveitis
Br. J. Ophthalmol.,
March 1, 2002;
86(3):
290 - 295.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Mansfield, V. Hinkovska-Galcheva, S. S. Carey, J. A. Shayman, and L. A. Boxer
Regulation of polymorphonuclear leukocyte degranulation and oxidant production by ceramide through inhibition of phospholipase D
Blood,
February 15, 2002;
99(4):
1434 - 1441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-B. Michel
Contrasting Outcomes of Atheroma Evolution: Intimal Accumulation Versus Medial Destruction
Arterioscler. Thromb. Vasc. Biol.,
September 1, 2001;
21(9):
1389 - 1392.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M.-C. Dang, A. R. Cross, and B. M. Babior
Assembly of the neutrophil respiratory burst oxidase: A direct interaction between p67PHOX and cytochrome b558
PNAS,
March 13, 2001;
98(6):
3001 - 3005.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Almkvist, J. Faldt, C. Dahlgren, H. Leffler, and A. Karlsson
Lipopolysaccharide-Induced Gelatinase Granule Mobilization Primes Neutrophils for Activation by Galectin-3 and Formylmethionyl-Leu-Phe
Infect. Immun.,
February 1, 2001;
69(2):
832 - 837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. M. Pruijt, W. E. Fibbe, L. Laterveer, R. A. Pieters, I. J. D. Lindley, L. Paemen, S. Masure, R. Willemze, and G. Opdenakker
Prevention of interleukin-8-induced mobilization of hematopoietic progenitor cells in rhesus monkeys by inhibitory antibodies against the Metalloproteinase gelatinase B (MMP-9)
PNAS,
September 14, 1999;
96(19):
10863 - 10868.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. DiMartino and R. R. Kew
Initial Characterization of the Vitamin D Binding Protein (Gc-Globulin) Binding Site on the Neutrophil Plasma Membrane: Evidence for a Chondroitin Sulfate Proteoglycan
J. Immunol.,
August 15, 1999;
163(4):
2135 - 2142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Karlsson
Wheat Germ Agglutinin Induces NADPH-Oxidase Activity in Human Neutrophils by Interaction with Mobilizable Receptors
Infect. Immun.,
July 1, 1999;
67(7):
3461 - 3468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. F. Gibbs, T. P. Shanley, R. L. Warner, H. S. Murphy, J. Varani, and K. J. Johnson
Role of Matrix Metalloproteinases in Models of Macrophage-Dependent Acute Lung Injury . Evidence for Alveolar Macrophage as Source of Proteinases
Am. J. Respir. Cell Mol. Biol.,
June 1, 1999;
20(6):
1145 - 1154.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. Tz. Hinkovska-Galcheva, L. A. Boxer, P. J. Mansfield, D. Harsh, A. Blackwood, and J. A. Shayman
The Formation of Ceramide-1-phosphate during Neutrophil Phagocytosis and Its Role in Liposome Fusion
J. Biol. Chem.,
December 11, 1998;
273(50):
33203 - 33209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Hinkovska-Galcheva, L. Kjeldsen, P. J. Mansfield, L. A. Boxer, J. A. Shayman, and S. J. Suchard
Activation of a Plasma Membrane-Associated Neutral Sphingomyelinase and Concomitant Ceramide Accumulation During IgG-Dependent Phagocytosis in Human Polymorphonuclear Leukocytes
Blood,
June 15, 1998;
91(12):
4761 - 4769.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Karlsson, P. Follin, H. Leffler, and C. Dahlgren
Galectin-3 Activates the NADPH-Oxidase in Exudated but not Peripheral Blood Neutrophils
Blood,
May 1, 1998;
91(9):
3430 - 3438.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Sorensen, K. Arnljots, J. B. Cowland, D. F. Bainton, and N. Borregaard
The Human Antibacterial Cathelicidin, hCAP-18, Is Synthesized in Myelocytes and Metamyelocytes and Localized to Specific Granules in Neutrophils
Blood,
October 1, 1997;
90(7):
2796 - 2803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Nagaoka, Y. Tsutsumi-Ishii, S. Yomogida, and T. Yamashita
Isolation of cDNA Encoding Guinea Pig Neutrophil Cationic Antibacterial Polypeptide of 11 kDa (CAP11) and Evaluation of CAP11 mRNA Expression during Neutrophil Maturation
J. Biol. Chem.,
September 5, 1997;
272(36):
22742 - 22750.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Borregaard and J. B. Cowland
Granules of the Human Neutrophilic Polymorphonuclear Leukocyte
Blood,
May 15, 1997;
89(10):
3503 - 3521.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Le Cabec, J. Calafat, and N. Borregaard
Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells
Blood,
March 15, 1997;
89(6):
2113 - 2121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Brumell, C. K. Chan, J. Butler, N. Borregaard, K. A. Siminovitch, S. Grinstein, and G. P. Downey
Regulation of Src Homology 2-containing Tyrosine Phosphatase 1during Activation of Human Neutrophils. ROLE OF PROTEIN KINASE C
J. Biol. Chem.,
January 10, 1997;
272(2):
875 - 882.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nanda, J. H. Brumell, T. Nordstrom, L. Kjeldsen, H. Sengelov, N. Borregaard, O. D. Rotstein, and S. Grinstein
Activation of Proton Pumping in Human Neutrophils Occurs by Exocytosis of Vesicles Bearing Vacuolar-type H+-ATPases
J. Biol. Chem.,
July 5, 1996;
271(27):
15963 - 15970.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kang, J. Yi, A. Guo, X. Wang, C. M. Overall, W. Jiang, R. Elde, N. Borregaard, and D. Pei
Subcellular Distribution and Cytokine- and Chemokine-regulated Secretion of Leukolysin/MT6-MMP/MMP-25 in Neutrophils
J. Biol. Chem.,
June 8, 2001;
276(24):
21960 - 21968.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Kruger, T. Fukushima, V. Cherepanov, N. Borregaard, C. Loeve, C. Shek, K. Sharma, A. K. Tanswell, C.-W. Chow, and G. P. Downey
Protein-tyrosine Phosphatase MEG2 Is Expressed by Human Neutrophils. LOCALIZATION TO THE PHAGOSOME AND ACTIVATION BY POLYPHOSPHOINOSITIDES
J. Biol. Chem.,
January 18, 2002;
277(4):
2620 - 2628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
