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Lymphoid and myeloid differentiation of single human CD34+, HLA-DR+, CD38-
hematopoietic stem cells
S Huang and LW Terstappen
Becton Dickinson Immunocytometry Systems, San Jose, CA 95131.
Multilineage differentiation of human fetal bone marrow CD34+ cell subsets
was examined using a single-cell liquid culture assay. Four CD34+ cell
populations, ie, (1) CD38-, HLA-DR+, (2) CD38-, HLA-DR-, (3) CD38+,
HLA-DR-, and (4) CD38+, HLA-DR+ cells, were sorted as single cells into
96-well flat-bottom culture plates containing long-term culture medium
supplemented with interleukin-3, interleukin-6, stem cell factor (SCF),
granulocyte-macrophage colony-stimulating factor, erythropoietin, basic
fibroblast growth factor (bFGF), and insulin-like growth factor-1 (IGF-1).
Single CD34+, CD38-, HLA-DR+ cells had the highest replating efficiency as
well as the highest replating efficiency. The cellular composition of the
single-cell progeny was studied by morphologic and/or flow cytometric
examination. Only the progeny of single CD34+ cells that lacked CD38 could
give rise to each of the hematopoietic cell lineages. The expansion of the
progeny of single CD34+, CD38-, HLA-DR+ cells was examined in more detail
and showed three clearly distinguishable growth patterns: 28% (SD, +/- 10%;
n = 14) of the single cells formed cell clusters/colonies; 9% (SD, +/- 4%;
n = 14) formed dispersed cells; and 11% (SD, +/- 6%; n = 14) gave rise to a
mixture of cell clusters and dispersed cells. The dispersed cell growth
pattern was reduced when SCF or bFGF and IGF-1 was absent in the growth
factor cocktail. The replating ability of the dispersed cells was
considerably larger than that of cells with other growth patterns, in that
76% of the cells that gave rise to dispersed cells and 54% of the cells
that gave rise to dispersed cells as well as cell clusters gave rise to a
second generation, but only 7% of the cells that gave rise to cell clusters
gave rise to a second generation. The second generation of cells continued
to produce third and fourth generations after repetitive replating, except
for the replated cells from cell clusters. In contrast with the
first-generation progeny, SCF did not have an influence on the replating
ability of the cells. Only in the progeny of single CD34+, CD38-, HLA-DR+
cells that gave rise to dispersed cells was each of the hematopoietic cell
lineages found, ie, B lymphocytes, neutrophils, monocytes, macrophages,
osteoclasts, basophils/mast cells, eosinophils, erythrocytes,
megakaryocytes, and platelets.(ABSTRACT TRUNCATED AT 400 WORDS)
Volume 83,
Issue 6,
pp. 1515-1526,
03/15/1994
Copyright © 1994 by The American Society of Hematology
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[Full Text]
[PDF]
|
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|
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December 15, 1998;
92(12):
4509 - 4520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Peters, S. Leyvraz, and L. Perey
Apoptotic Regulation in Primitive Hematopoietic Precursors
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92(6):
2041 - 2052.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Opie, L. E. Shields, and R. G. Andrews
Cell-Surface Antigen Expression in Early and Term Gestation Fetal Hematopoietic Progenitor Cells
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16(5):
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[Abstract]
[Full Text]
|
|
|
|
|
|
|
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Mice Deficient for the Ecto-Nicotinamide Adenine Dinucleotide Glycohydrolase CD38 Exhibit Altered Humoral Immune Responses
Blood,
August 15, 1998;
92(4):
1324 - 1333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sawai, K. Koike, T. Higuchi, K. Ogami, and M. Oda
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Blood,
June 1, 1998;
91(11):
4065 - 4073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. J. Elwood, H. Zogos, D. S. Pereira, J. E. Dick, and C. G. Begley
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May 15, 1998;
91(10):
3756 - 3765.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
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The Effect of alpha 4beta 1-Integrin Binding Sequences of Fibronectin on Growth of Cells From Human Hematopoietic Progenitors
Blood,
May 1, 1998;
91(9):
3230 - 3238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Dao, A. J. Shah, G. M. Crooks, and J. A. Nolta
Engraftment and Retroviral Marking of CD34+ and CD34+CD38- Human Hematopoietic Progenitors Assessed in Immune-Deficient Mice
Blood,
February 15, 1998;
91(4):
1243 - 1255.
[Abstract]
[Full Text]
[PDF]
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