|
|
 Previous Article | Table of Contents | Next Article 
The 87-kD A gamma-globin enhancer-binding protein is a product of the
HOXB2(HOX2H) locus
PK Sengupta, DE Lavelle and J DeSimone
Department of Medicine, University of Illinois, Chicago.
Developmental regulation of globin gene expression may be controlled by
developmental stage-specific nuclear proteins that influence interactions
between the locus control region and local regulatory sequences near
individual globin genes. We previously isolated an 87-kD nuclear protein
from K562 cells that bound to DNA sequences in the beta- globin locus
control region, gamma-globin promoter, and A gamma-globin enhancer. The
presence of this protein in fetal globin-expressing cells and its absence
in adult globin-expressing cells suggested that it may be a developmental
stage-specific factor. A lambda gt11 K562 cDNA clone encoding a portion of
the HOXB2 (formerly HOX2H) homeobox gene was isolated on the basis of the
ability of its beta-galactosidase fusion protein to bind to the same DNA
sequences as the 87-kD K562 protein. Because no other relationship had been
established between the 87-kD K562 protein and the HOXB2 protein other than
their ability to bind ot the same DNA sequences, we have investigated
whether the two proteins are related antigenically. Our data show that
antisera produced against the HOXB2-beta-gal fusion protein and a synthetic
HOXB2 decapeptide react specifically with an 87-kD protein from K562
nuclear extract, showing that the 87-kD K562 nuclear protein is a product
of the HOXB2 locus, and is the first demonstration of cellular HOXB2
protein.
Volume 83,
Issue 5,
pp. 1420-1427,
03/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. Prabagaran, A.H. Bandivdekar, V. Dighe, and V.P. Raghavan
HOXBES2: A Novel Epididymal HOXB2 Homeoprotein and Its Domain-Specific Association with Spermatozoa
Biol Reprod,
February 1, 2007;
76(2):
314 - 326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Ferrell, S. T. Dorsam, H. Ohta, R. K. Humphries, M. K. Derynck, C. Haqq, C. Largman, and H. J. Lawrence
Activation of Stem-Cell Specific Genes by HOXA9 and HOXA10 Homeodomain Proteins in CD34+ Human Cord Blood Cells
Stem Cells,
May 1, 2005;
23(5):
644 - 655.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Guazzi, M. L. Pintonello, A. Vigano, and E. Boncinelli
Regulatory Interactions between the Human HOXB1, HOXB2, and HOXB3 Proteins and the Upstream Sequence of the Otx2 Gene in Embryonal Carcinoma Cells
J. Biol. Chem.,
May 1, 1998;
273(18):
11092 - 11099.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N.A. Roberts, J.A. Sloane-Stanley, J.A. Sharpe, S.J. Stanworth, and W.G. Wood
Globin Gene Switching in Transgenic Mice Carrying HS2-Globin Gene Constructs
Blood,
January 15, 1997;
89(2):
713 - 723.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Lawrence, G Sauvageau, R. Humphries, and C Largman
The role of HOX homeobox genes in normal and leukemic hematopoiesis
Stem Cells,
May 1, 1996;
14(3):
281 - 291.
[Abstract]
|
|
|
|
|
|
|
C. E. Jackson, D. O' Neill, and A. Bank
Nuclear Factor Binding Sites in Human beta Globin IVS2
J. Biol. Chem.,
November 24, 1995;
270(47):
28448 - 28456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Vieille-Grosjean and P. Huber
Transcription Factor GATA-1 Regulates Human HOXB2 Gene Expression in Erythroid Cells
J. Biol. Chem.,
March 3, 1995;
270(9):
4544 - 4550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
