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Stem cell factor and basic fibroblast growth factor are synergistic in
augmenting committed myeloid progenitor cell growth
JL Gabrilove, K White, Z Rahman and EL Wilson
Laboratory of Leukocyte Biology, Sloan Kettering Institute, New York, NY
10021.
Stem cell factor (SCF) and basic fibroblast growth factor (bFGF) are
hematopoietic cytokines produced by bone marrow stromal cells. It is known
that, although SCF and bFGF have limited clonogenic activity on their own,
they can augment colony-stimulating factor (CSF)-mediated progenitor cell
growth. Because these factors are both sequestered by stromal cells, we
examined their interaction on progenitor cell growth in conjunction with
granulocyte-macrophage-CSF (GM-CSF). In this study, we show that clonogenic
growth derived from low-density bone marrow cells stimulated by GM-CSF is
significantly augmented (P < .001) in the presence of maximal (100
ng/mL) concentrations of SCF in combination with 100 ng/mL of bFGF. When
CD34+ cells are used, the synergistic effect of bFGF and SCF for
GM-CSF-mediated progenitor cell growth is further increased, resulting in
as much as a sevenfold increase in detectable colony-forming units
granulocyte-macrophage (P < .001). These data suggest that the
synergistic activity of bFGF and SCF is mediated directly on hematopoietic
precursors. These observations suggest that bFGF and SCF, concentrated
locally on stromal cell surfaces, might interact in concert with other
hematopoietic cytokines to regulate stem cell proliferation and
differentiation in hematopoietic niches in the bone marrow.
Volume 83,
Issue 4,
pp. 907-910,
02/15/1994
Copyright © 1994 by The American Society of Hematology
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