|
|
 Previous Article | Table of Contents | Next Article 
c-kit Gene was not transcribed in cultured mast cells of mast cell-
deficient Wsh/Wsh mice that have a normal number of erythrocytes and a
normal c-kit coding region
T Tono, T Tsujimura, U Koshimizu, T Kasugai, S Adachi, K Isozaki, S Nishikawa, M Morimoto, Y Nishimune and S Nomura
Department of Pathology, Medical School, Osaka University, Suita, Japan.
The Wsh is a mutant allele at the W (c-kit) locus of mice. Mice of Wsh/Wsh
genotype have white hairs and black eyes. Although adult C57BL/6-Wsh/Wsh
mice were not anemic, they showed a remarkable depletion of mast cells.
Most homozygous or double heterozygous mutant mice at the W (c-kit) locus,
of which mast-cell depletion was comparable to that of Wsh/Wsh mice, are
deficient in germ cells. However, male and female Wsh/Wsh mice have an
appreciable number of germ cells in their gonads. We investigated the
mechanism of specific depletion of mast cells in Wsh/Wsh mice. Cultured
mast cells (CMC) derived from the spleen of Wsh/Wsh mice neither attached
to normal (+/+) fibroblasts nor survived in the coculture with +/+
fibroblasts. The c-kit messenger RNA (mRNA) was strongly expressed in +/+
CMC, but not detectable in Wsh/Wsh CMC. Despite the lack of c-kit mRNA in
Wsh/Wsh CMC, the c-kit mRNA was normally detectable in the cerebellum and
weakly detectable in the testis and spleen of Wsh/Wsh mice. No significant
changes were found in the nucleotide sequence of the c-kit transcripts
obtained from the cerebellum of Wsh/Wsh mice. Development of mast cells,
erythrocytes, and germ cells in Wsh/Wsh mice appeared to be parallel with
the magnitude of the c-kit gene expression in each cell type.
Volume 80,
Issue 6,
pp. 1448-1453,
09/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. E. Sutherland, J. S. Olsen, A. McKinstry, S. A. Villalta, and P. J. Wolters
Mast Cell IL-6 Improves Survival from Klebsiella Pneumonia and Sepsis by Enhancing Neutrophil Killing
J. Immunol.,
October 15, 2008;
181(8):
5598 - 5605.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Zabel, S. Nakae, L. Zuniga, J.-Y. Kim, T. Ohyama, C. Alt, J. Pan, H. Suto, D. Soler, S. J. Allen, et al.
Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis
J. Exp. Med.,
September 29, 2008;
205(10):
2207 - 2220.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Grimbaldeston, C.-C. Chen, A. M. Piliponsky, M. Tsai, S.-Y. Tam, and S. J. Galli
Mast Cell-Deficient W-sash c-kit Mutant KitW-sh/W-sh Mice as a Model for Investigating Mast Cell Biology in Vivo
Am. J. Pathol.,
September 1, 2005;
167(3):
835 - 848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S Komatsu, M B Grisham, J M Russell, and D N Granger
Enhanced mucosal permeability and nitric oxide synthase activity in jejunum of mast cell deficient mice
Gut,
November 1, 1997;
41(5):
636 - 641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T Hirata, E Morii, M Morimoto, T Kasugai, T Tsujimura, S Hirota, Y Kanakura, S Nomura, and Y Kitamura
Stem cell factor induces outgrowth of c-kit-positive neurites and supports the survival of c-kit-positive neurons in dorsal root ganglia of mouse embryos
Development,
January 9, 1993;
119(1):
49 - 56.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R Duttlinger, K Manova, T. Chu, C Gyssler, A. Zelenetz, R. Bachvarova, and P Besmer
W-sash affects positive and negative elements controlling c-kit expression: ectopic c-kit expression at sites of kit-ligand expression affects melanogenesis
Development,
January 7, 1993;
118(3):
705 - 717.
[Abstract]
[PDF]
|
|
|
|
|
