|
|
 Previous Article | Table of Contents | Next Article 
Human B-cell interleukin-10: B-cell lines derived from patients with
acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively
secrete large quantities of interleukin-10 [see comments]
D Benjamin, TJ Knobloch and MA Dayton
Division of Hematology and Oncology, Arthur G. James Cancer Hospital and
Research Institute, Ohio State University, Columbus 43210-1228.
A recent addition to the lymphokine network is human IL-10 (hIL-10). This
novel lymphokine has striking homology to BCRF1 protein, the product of a
previously uncharacterized open-reading frame in the Epstein-Barr virus
(EBV) genome. To date, IL-10 expression has been described in several T
clones induced with anti-CD3 and phorbol myristate acetate (PMA), in
monocytes stimulated with lipopolysaccharide (LPS), and in murine B-cell
lymphomas. We sought to determine whether human B cells express hIL-10 and,
if so, its relationship to EBV and to other B-cell lymphokines. We studied
21 EBV- positive B-cell lines derived from patients with acquired
immunodeficiency syndrome (AIDS) and Burkitt's lymphoma (n = 6), American
Burkitt's (n = 3), African Burkitt's (n = 5), and normal lymphoblastoid
cell lines (n = 7), in comparison with seven EBV- negative cell lines. All
cell lines were activated with the tumor promoters PMA and teleocidin and
were studied by Northern blot analysis, reverse transcription-polymerase
chain reaction (RT-PCR), and enzyme-linked immunoadsorbent assay (ELISA).
We demonstrated that EBV- positive cell lines derived from patients with
American Burkitt's lymphoma, and especially those from patients with AIDS,
constitutively express large quantities of hIL-10 by Northern blot analysis
and ELISA (range, 3,101 to 25,915 pg/mL), and that both teleocidin and PMA
induce hIL-10 in these cell lines. In contrast, six of seven EBV-negative
cell lines did not express hIL-10 even by RT-PCR, and hIL-10 was not
triggered by PMA or teleocidin. To assure that the 350 bp amplified by PCR
was hIL-10 and not BCRF1, we used PCR primers, which do not amplify a
fragment from plasmid templates containing BCRF1. Cloning and sequencing of
the 350 bp product also demonstrated that B-cell IL-10 is identical to
hIL-10 from the T-cell clone B21. Correlation of hIL-10 with other B-cell
lymphokines secreted by these B-cell lines demonstrated that hIL-10
secretor cell lines also constitutively secrete or can be induced to
secrete IL-6, although to a much lesser amount. Since both lymphokines
influence B-cell growth and differentiation, we suggest that hIL-10 may
contribute to the polyclonal B-cell activation and hyperglobulinemia seen
in AIDS patients. Finally, several reports support the hypothesis that EBV
is an important cofactor in the development of human immunodeficiency virus
type 1 (HIV-1)-related B-cell lymphomas. Detection of large quantities of
hIL-10 in B-cell lines derived from AIDS patients, the close association
between EBV and hIL-10 shown in this report, and the ability of BCRF1 to
capture hIL-10 activities, make hIL-10/BCRF1 an attractive candidate as a
factor causing B-cell growth and immortalization in patients with AIDS and
B-cell lymphomas.
Volume 80,
Issue 5,
pp. 1289-1298,
09/01/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Lizee, L. G. Radvanyi, W. W. Overwijk, and P. Hwu
Improving Antitumor Immune Responses by Circumventing Immunoregulatory Cells and Mechanisms.
Clin. Cancer Res.,
August 15, 2006;
12(16):
4794 - 4803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kalies, M. Blessenohl, J. Nietsch, and J. Westermann
T Cell Zones of Lymphoid Organs Constitutively Express Th1 Cytokine mRNA: Specific Changes during the Early Phase of an Immune Response
J. Immunol.,
January 15, 2006;
176(2):
741 - 749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Rothwell, J. R. Young, R. Zoorob, C. A. Whittaker, P. Hesketh, A. Archer, A. L. Smith, and P. Kaiser
Cloning and Characterization of Chicken IL-10 and Its Role in the Immune Response to Eimeria maxima
J. Immunol.,
August 15, 2004;
173(4):
2675 - 2682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Lech-Maranda, L. Baseggio, J. Bienvenu, C. Charlot, F. Berger, D. Rigal, K. Warzocha, B. Coiffier, and G. Salles
Interleukin-10 gene promoter polymorphisms influence the clinical outcome of diffuse large B-cell lymphoma
Blood,
May 1, 2004;
103(9):
3529 - 3534.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Nelson, A. M. Levine, and L. Bernstein
Reproductive Factors and Risk of Intermediate- or High-Grade B-Cell Non-Hodgkin's Lymphoma in Women
J. Clin. Oncol.,
March 1, 2001;
19(5):
1381 - 1387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Fayad, M. J. Keating, J. M. Reuben, S. O'Brien, B.-N. Lee, S. Lerner, and R. Kurzrock
Interleukin-6 and interleukin-10 levels in chronic lymphocytic leukemia: correlation with phenotypic characteristics and outcome
Blood,
January 1, 2001;
97(1):
256 - 263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takahashi, T. Fujita, and A. Yamamoto
Role of cyclooxygenase-2 in Helicobacter pylori- induced gastritis in Mongolian gerbils
Am J Physiol Gastrointest Liver Physiol,
October 1, 2000;
279(4):
G791 - G798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Oksenhendler, G. Carcelain, Y. Aoki, E. Boulanger, A. Maillard, J.-P. Clauvel, and F. Agbalika
High levels of human herpesvirus 8 viral load, human interleukin-6, interleukin-10, and C reactive protein correlate with exacerbation of multicentric Castleman disease in HIV-infected patients
Blood,
September 15, 2000;
96(6):
2069 - 2073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. K. Akpek, C. S. Foster, R. R. Buggage, S. M. Whitcup, R. B. Nussenblatt, and C.-C. Chan
Primary Intraocular Lymphoma With a Low Interleukin 10 to Interleukin 6 Ratio and Heterogeneous IgH Gene Arrangement
Arch Ophthalmol,
May 1, 2000;
118(5):
731 - 732.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Samandari, K. L. Kotloff, G. A. Losonsky, W. D. Picking, P. J. Sansonetti, M. M. Levine, and M. B. Sztein
Production of IFN-{gamma} and IL-10 to Shigella Invasins by Mononuclear Cells from Volunteers Orally Inoculated with a Shiga Toxin-Deleted Shigella dysenteriae Type 1 Strain
J. Immunol.,
February 15, 2000;
164(4):
2221 - 2232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Loercher, M. A. Nash, J. J. Kavanagh, C. D. Platsoucas, and R. S. Freedman
Identification of an IL-10-Producing HLA-DR-Negative Monocyte Subset in the Malignant Ascites of Patients with Ovarian Carcinoma That Inhibits Cytokine Protein Expression and Proliferation of Autologous T Cells
J. Immunol.,
December 1, 1999;
163(11):
6251 - 6260.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Kossakowska, D. R. Edwards, C. Prusinkiewicz, M. C. Zhang, D. Guo, S. J. Urbanski, T. Grogan, L. A. Marquez, and A. Janowska-Wieczorek
Interleukin-6 Regulation of Matrix Metalloproteinase (MMP-2 and MMP-9) and Tissue Inhibitor of Metalloproteinase (TIMP-1) Expression in Malignant Non-Hodgkin's Lymphomas
Blood,
September 15, 1999;
94(6):
2080 - 2089.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.T. Bejarano and M.G. Masucci
Interleukin-10 Abrogates the Inhibition of Epstein-Barr Virus-Induced B-Cell Transformation by Memory T-Cell Responses
Blood,
December 1, 1998;
92(11):
4256 - 4262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. W. Redford, A.-K. Yi, C. T. Ward, and A. M. Krieg
Cyclosporin A Enhances IL-12 Production by CpG Motifs in Bacterial DNA and Synthetic Oligodeoxynucleotides
J. Immunol.,
October 15, 1998;
161(8):
3930 - 3935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-L. Boulland, V. Meignin, K. Leroy-Viard, C. Copie-Bergman, J. Briere, R. Touitou, P. Kanavaros, and P. Gaulard
Human Interleukin-10 Expression in T/Natural Killer-Cell Lymphomas : Association with Anaplastic Large Cell Lymphomas and Nasal Natural Killer-Cell Lymphomas
Am. J. Pathol.,
October 1, 1998;
153(4):
1229 - 1237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Petersson, J. Charo, F. Salazar-Onfray, G. Noffz, M. Mohaupt, Z. Qin, G. Klein, T. Blankenstein, and R. Kiessling
Constitutive IL-10 Production Accounts for the High NK Sensitivity, Low MHC Class I Expression, and Poor Transporter Associated with Antigen Processing (TAP)-1/2 Function in the Prototype NK Target YAC-1
J. Immunol.,
September 1, 1998;
161(5):
2099 - 2105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Autschbach, J. Braunstein, B. Helmke, I. Zuna, G. Schurmann, Z. I. Niemir, R. Wallich, H. F. Otto, and S. C. Meuer
In Situ Expression of Interleukin-10 in Noninflamed Human Gut and in Inflammatory Bowel Disease
Am. J. Pathol.,
July 1, 1998;
153(1):
121 - 130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Asou, J. W. Said, R. Yang, R. Munker, D. J. Park, N. Kamada, and H. P. Koeffler
Mechanisms of Growth Control of Kaposi's Sarcoma-Associated Herpes Virus-Associated Primary Effusion Lymphoma Cells
Blood,
April 1, 1998;
91(7):
2475 - 2481.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Dorfman, J. L. Schultze, A. Shahsafaei, S. Michalak, J. G. Gribben, G. J. Freeman, G. S. Pinkus, and L. M. Nadler
In Vivo Expression of B7-1 and B7-2 By Follicular Lymphoma Cells Can Prevent Induction of T-Cell Anergy But Is Insufficient to Induce Significant T-Cell Proliferation
Blood,
December 1, 1997;
90(11):
4297 - 4306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Gaidano, A. Carbone, C. Pastore, D. Capello, A. Migliazza, A. Gloghini, S. Roncella, M. Ferrarini, G. Saglio, and R. Dalla-Favera
Frequent Mutation of the 5' Noncoding Region of the BCL-6 Gene in Acquired Immunodeficiency Syndrome-Related Non-Hodgkin's Lymphomas
Blood,
May 15, 1997;
89(10):
3755 - 3762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
