|
|
 Previous Article | Table of Contents | Next Article 
Enhanced expression of interleukin-3 and granulocyte-macrophage colony-
stimulating factor receptor subunits in murine hematopoietic cells
stimulated with hematopoietic growth factors
E Liboi, P Jubinsky, NC Andrews, DG Nathan and B Mathey-Prevot
Division of Pediatric Hematology and Oncology, Children's Hospital, Boston,
MA.
AIC2A and AIC2B are closely related genes encoding components of the
receptors for murine interleukin-3 (IL-3) (AIC2A) and granulocyte-
macrophage colony-stimulating factor (GM-CSF) and IL-5 (AIC2B). We have
studied the parallel regulation of expression of these genes in erythroid
and myeloid progenitor cell lines. AIC2A and AIC2B transcription was
transiently induced in these cells in response to a variety of
hematopoietic growth factors, including erythropoietin (EPO), monocyte-CSF,
IL-3, GM-CSF, and stem cell factor (SCF or kit ligand). Run-on assays
established that the increase occurred mainly at the transcriptional level.
Immunoprecipitation experiments confirmed that the increase in messenger
RNA expression resulted in augmented synthesis of both AIC2A and AIC2B
proteins, and binding studies further showed these proteins to be
functional. We observed a fourfold increase in low-affinity IL-3 sites in
an erythroid precursor cell line stimulated with EPO, and a threefold
increase in GM-CSF high-affinity sites in a myeloid cell line stimulated
with IL-3. In addition, we showed that the increase in the IL-3 receptor
chain AIC2A in the erythroid precursor cell line correlated with the
ability of IL-3 to exert a cooperative effect with EPO in the induction of
beta-globin in these cells.
Volume 80,
Issue 5,
pp. 1183-1189,
09/01/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. B. van Dijk, B. Baltus, E. Caldenhoven, H. Handa, J. A.M. Raaijmakers, J.-W. J. Lammers, L. Koenderman, and R. P. de Groot
Cloning and Characterization of the Human Interleukin-3 (IL-3)/IL-5/ Granulocyte-Macrophage Colony-Stimulating Factor Receptor beta c Gene: Regulation by Ets Family Members
Blood,
November 15, 1998;
92(10):
3636 - 3646.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pless, K. Norga, M. Carroll, M. H. Heim, A. D. D'Andrea, and B. Mathey-Prevot
Receptors That Induce Erythroid Differentiation of Ba/F3 Cells: Structural Requirements and Effect on STAT5 Binding
Blood,
May 1, 1997;
89(9):
3175 - 3185.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.-E. Zhang, P. Zhang, N.-d. Wang, C. J. Hetherington, G. J. Darlington, and D. G. Tenen
Absence of granulocyte colony-stimulating factor signaling and neutrophil development in CCAAT enhancer binding protein alpha -deficient mice
PNAS,
January 21, 1997;
94(2):
569 - 574.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Cattaneo, C. De Fraja, L. Conti, B. Reinach, L. Bolis, S. Govoni, and E. Liboi
Activation of the JAK/STAT Pathway Leads to Proliferation of ST14A Central Nervous System Progenitor Cells
J. Biol. Chem.,
September 20, 1996;
271(38):
23374 - 23379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Tilbrook, T. Bittorf, S. J. Busfield, D. Chappell, and S. P. Klinken
Disrupted Signaling in a Mutant J2E Cell Line That Shows Enhanced Viability, but Does Not Proliferate or Differentiate, with Erythropoietin
J. Biol. Chem.,
February 16, 1996;
271(7):
3453 - 3459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
