|
|
 Previous Article | Table of Contents | Next Article 
Shear rate-dependent impairment of thrombus growth on collagen in
nonanticoagulated blood from patients with von Willebrand disease and
hemophilia A
E Fressinaud, KS Sakariassen, C Rothschild, HR Baumgartner and D Meyer
Centre de Transfusion, Angers, France.
Thrombus formation on collagen fibrils was quantified at venous (100/s) and
arterial (650/s and 2,600/s) wall shear rates in blood from patients with
various subtypes of von Willebrand disease (vWD) and with hemophilia A
(HA). Nonanticoagulated blood was drawn directly from an antecubital vein
over purified type III collagen fibrils exposed in parallel-plate perfusion
chambers. Blood-collagen interactions were differentiated and quantified by
morphometry as platelet adhesion, thrombus height, thrombus volume, and
deposition of fibrin strands. Sixteen patients with vWD, including four
type III, six type I, four type IIA, and two type IIB, were compared with
26 normal subjects and nine patients with HA, including six severe HA and
three mild HA. Platelet adhesion and thrombus formation at 2,600/s were
significantly decreased in blood from patients with vWD type III, IIA, and
IIB, but not in blood from patients with type I and in HA. The abnormal
thrombus formation was apparently not related to the decreased levels of
factor VIII (F.VIII), because thrombus height and volume were normal in
severe and mild HA. Thrombus formation at 650/s was also significantly
decreased in patients with vWD type III, IIA, and IIB and slightly reduced
in type I. Significant reduction in thrombus volume and height was also
observed in blood from patients with severe HA, but not in mild HA.
Thrombus dimensions were not affected at 100/s in the vWD subtypes.
However, significantly decreased thrombus height and virtually absent
fibrin deposition were observed in blood from patients with severe HA.
Apparently, F.VIII supports thrombus formation at low and intermediate
shear conditions, presumably through the generation of thrombin. In
contrast, von Willebrand factor (vWF) mediates not only platelet adhesion,
but also thrombus formation at intermediate and high shear rates. Thus, the
relative contribution of coagulation (F.VIII) and platelet function (vWF)
in thrombus formation appears to be shear rate dependent, but having
optimal effects at different shear conditions.
Volume 80,
Issue 4,
pp. 988-994,
08/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. J. Hathcock
Flow Effects on Coagulation and Thrombosis
Arterioscler. Thromb. Vasc. Biol.,
August 1, 2006;
26(8):
1729 - 1737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Andre, P. Hainaud, C. Bal dit Sollier, L. I. Garfinkel, J. P. Caen, and L. O. Drouet
Relative Involvement of GPIb/IX-vWF Axis and GPIIb/IIIa in Thrombus Growth at High Shear Rates in the Guinea Pig
Arterioscler. Thromb. Vasc. Biol.,
May 1, 1997;
17(5):
919 - 924.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Kirchhofer, M. A. Riederer, and H. R. Baumgartner
Specific Accumulation of Circulating Monocytes and Polymorphonuclear Leukocytes on Platelet Thrombi in a Vascular Injury Model
Blood,
February 15, 1997;
89(4):
1270 - 1278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Orvim, R. M. Barstad, G. P. Vlasuk, and K. S. Sakariassen
Effect of Selective Factor Xa Inhibition on Arterial Thrombus Formation Triggered by Tissue Factor/Factor VIIa or Collagen in an Ex Vivo Model of Shear-Dependent Human Thrombogenesis
Arterioscler. Thromb. Vasc. Biol.,
December 1, 1995;
15(12):
2188 - 2194.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. E. Roald, T. Lyberg, H. Dedichen, M. Hamers, P. Kierulf, A.-B. Westvik, and K. S. Sakariassen
Collagen-Induced Thrombus Formation in Flowing Nonanticoagulated Human Blood From Habitual Smokers and Nonsmoking Patients With Severe Peripheral Atherosclerotic Disease
Arterioscler. Thromb. Vasc. Biol.,
January 1, 1995;
15(1):
128 - 132.
[Abstract]
[Full Text]
|
|
|
|
|
