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Blood platelets stimulate the expression of chondroitin sulfate
proteoglycan in human monocytes
L Uhlin-Hansen, D Langvoll, T Wik and SO Kolset
Department of Biochemistry, University of Tromso, Norway.
Mononuclear phagocytes synthesize chondroitin sulfate proteoglycan (CSPG),
which is constitutively secreted. Because mononuclear phagocytes are known
to interact with blood platelets, the effect of platelets on the release of
CSPG in cultured human monocytes was investigated. After 6 days in vitro,
the monocytes were supplied with fresh medium with different additions and
subsequently exposed to [35S]sulfate for 24 hours before the medium
fractions were harvested and analyzed for content of [35S]CSPG. Indirect
evidence for the release of stimulatory factors from blood platelets was
found when the addition of medium containing 50% serum made from
platelet-rich plasma increased the expression of [35S]CSPG almost sevenfold
compared with serum-free medium, whereas medium containing 50% serum made
from platelet-depleted plasma increased the expression of [35S]CSPG about
fourfold. Further, direct evidence for the stimulatory effect of platelets
was found as the addition of autologous platelets to serum- free medium
increased the expression of [35S]CSPG about threefold, and addition of
supernatant from a corresponding number of thrombin- stimulated platelets
was almost as efficient. The effect of five different platelet-derived
factors (which are all present in serum) was investigated. Both
platelet-derived growth factor (PDGF), platelet factor 4 (PF 4), and
prostaglandin E2 (PGE2) used in physiologic concentrations were found to
stimulate the expression of [35S]CSPG twofold to threefold, whereas
transforming growth factor-beta had a slight inhibitory effect.
12-Hydroxyeicosatetraenoic acid had no significant effect on the expression
of [35S]CSPG. Further evidence for the stimulatory effect of PDGF, PF 4,
and PGE2 was found as serum depleted of these factors had significantly
less stimulatory effect than control serum. The increased incorporation of
[35S]sulfate into [35S]CSPG in cultures stimulated with serum or
platelet-derived factors was not due to differences in molecular size or
extent of sulfation of the proteoglycan molecules.
Volume 80,
Issue 4,
pp. 1058-1065,
08/15/1992
Copyright © 1992 by The American Society of Hematology
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