Simultaneous genotypic and immunophenotypic analysis of interphase cells
using dual-color fluorescence: a demonstration of lineage involvement in
polycythemia vera
CM Price, EJ Kanfer, SM Colman, N Westwood, AJ Barrett and MF Greaves
Leukaemia Research Fund Centre, Institute of Cancer Research, London, UK.
Fluorescent in situ hybridization has become a useful technique by which
chromosomal abnormalities may be shown in interphase cells. We present a
dual-fluorescence method whereby a chromosomal and immunophenotypic marker
can be visualized simultaneously in the same interphase cell. Two patients
with the myeloproliferative disorder polycythemia vera and trisomy for
chromosome 8 have been studied using this technique and selective
involvement of the myeloid and erythrocyte lineages has been shown by the
detection of the trisomy in immunophenotyped cells. Simultaneous analysis
of genotype and immunophenotype in individual cells from patients with
myeloproliferative disorders or leukemia may help identify the
developmental and lineage status of cells in which molecular alterations
have resulted in clonal advantage.
Volume 80,
Issue 4,
pp. 1033-1038,
08/15/1992
Copyright © 1992 by The American Society of Hematology
