OMA-AML-1: a leukemic myeloid cell line with CD34+ progenitor and CD15+
spontaneously differentiating cell compartments
SJ Pirruccello, JD Jackson, MS Lang, J DeBoer, S Mann, D Crouse, WP Vaughan, KA Dicke and JG Sharp
Department of Pathology and Microbiology, University of Nebraska Medical
Center, Omaha 68198.
OMA-AML-1 was established from a patient with acute myelomonocytic (M4)
leukemia at fifth relapse when blasts were greater than 85% CD34+, CD15- .
Leukemic cells were established in suspension culture and independently
grown as subcutaneous tumors in SCID mice. Cells growing in suspension
culture underwent differentiation by phenotypic and morphologic criteria.
In contrast, cells grown as subcutaneous solid tumors in SCID mice
maintained progenitor cell characteristics with high-density CD34
expression and lack of morphologic differentiation. A tendency toward
differentiation to CD15+, CD34- cells in vitro and self- renewal of CD34+,
CD15- cells in vivo was consistently demonstrated regardless of whether
cells were initially grown in vitro or in vivo. The cell line maintains
both a CD34+, CD15- progentitor cell pool and a non-overlapping, CD15+,
CD34- differentiating cell compartment after more than 1 year in continuous
culture. Cell cycle analysis and cloning experiments were consistent with
terminal differentiation occurring in the CD15+, CD34- population. The cell
line shows concentration- dependent proliferative responses to interleukin
(IL)-3, granulocyte- macrophage colony-stimulating factor (GM-CSF), and
IL-6, but not to granulocyte CSF (G-CSF). OMA-AML-1 appears to mimic
several features of normal myeloid hematopoiesis and should prove useful
for the study of normal and malignant myeloid differentiation.
Volume 80,
Issue 4,
pp. 1026-1032,
08/15/1992
Copyright © 1992 by The American Society of Hematology
