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Molecular cloning and analysis of in vivo expression of murine P- selectin
WE Sanders, RW Wilson, CM Ballantyne and AL Beaudet
Baylor College of Medicine, Department of Internal Medicine, Houston, TX
77030.
P-selectin (CD62) is a rapidly inducible cell surface adhesion molecule
that is expressed on platelets and endothelial cells and mediates their
interaction with leukocytes. In vitro studies have suggested that this
receptor may play an important role in hemostasis and in inflammatory
response to tissue injury. We report the molecular cloning and sequencing
of murine cDNA for P-selectin. The lectin, epidermal growth factor
(EGF)-like, transmembrane, and cytoplasmic domains are highly conserved
between mouse and human, with an overall amino acid identity of 79%. To
further investigate the biology of this adhesion molecule in vivo, we
analyzed mRNA levels for P-selectin in mice after injection with endotoxin.
Northern blot data indicate that the cellular response in vivo includes a
rapid increase in the level of mRNA, presumably for new synthesis of
P-selectin. The increase in mRNA is maximal at 4 hours, and turnover is
relatively rapid, with levels of RNA having decreased substantially by 6
hours following stimulation with endotoxin. After administration of
endotoxin, the highest levels of mRNA expression were detected in liver,
lung, kidney, and heart.
Volume 80,
Issue 3,
pp. 795-800,
08/01/1992
Copyright © 1992 by The American Society of Hematology
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