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Differentiation and functional activity of human eosinophilic cells from an
eosinophil HL-60 subline: response to recombinant hematopoietic growth
factors [published erratum appears in Blood 1992 Dec 1;80(11):2952]
I Fabian, M Lass, Y Kletter and DW Golde
Department of Histology and Cell Biology, Sackler School of Medicine, Tel
Aviv, Israel.
We studied the effect of hematopoietic growth factors (granulocyte-
macrophage colony-stimulating factor [GM-CSF], granulocyte [G]-CSF,
interleukin (IL)-1, IL-3, IL-5, IL-6, and macrophage [M]-CSF) on
differentiation and functional activity of human eosinophilic HL-60 cells
(Eos-HL-60) and compared them with effects on parental HL-60 promyelocytic
leukemia cells. Purified biosynthetic GM-CSF and IL-5 enhanced cell
proliferation and induced eosinophilic differentiation in the eosinophilic
subline in both liquid and agar cultures. IL-3 and IL- 6 stimulated cell
proliferation but had no effect on cell differentiation, whereas IL-1 and
G-CSF affected neither differentiation nor proliferation of Eos-HL-60 cells
under the conditions tested. GM-CSF-, IL-3-, and IL-5-treated Eos-HL-60
cells showed increased O2- production in response to phorbol esters (PMA),
enhanced phagocytosis of Candida albicans, and release of the enzymes
arylsulfatase, beta-glucuronidase and eosinophil peroxidase (EPO). The
degranulation of eosinophils induced by GM-CSF, IL-5, and IL-3 may have
relevance to the potential clinical toxicity of these hematopoietins, which
also stimulate eosinophilopoiesis. G-CSF had no effect on enzyme release,
oxidative metabolism, or phagocytic capacity of Eos-HL-60 cells. IL-5 did
not affect proliferation, differentiation, or enzyme release in
promyelocytic HL-60 cells. These results indicate the specificity of IL-5
for the eosinophil lineage, confirm the effects of GM-CSF and IL-3 on
eosinophilopoiesis and mature eosinophil function in a model system, and
indicate the absence of G-CSF and IL-1 stimulation of eosinophils. The
Eos-HL-60 line is a useful model for studying human eosinophil responses to
cytokines.
Volume 80,
Issue 3,
pp. 788-794,
08/01/1992
Copyright © 1992 by The American Society of Hematology
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