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Changes in cell surface antigen expressions during proliferation and
differentiation of human erythroid progenitors
N Okumura, K Tsuji and T Nakahata
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto,
Japan.
Cell surface antigen expression during proliferation and differentiation of
human erythroid progenitors was examined using a combination of sequential
micromanipulations of paired daughter cells derived from erythroid
burst-forming units (BFU-E) and immuno-staining with a panel of monoclonal
antibodies. Single hematopoietic progenitors were identified in
methylcellulose cultures containing human cord blood mononuclear cells and
micromanipulated individually to secondary culture. Paired daughter cells,
granddaughter cells, and subsequent generations, whose counterparts
produced erythroid bursts, were stained with various cytochemical and
immuno-alkaline phosphatase stainings. Most paired daughter cells of BFU-E
immunostained positively with anti- platelet glycoprotein(GP) IIb,
antiplatelet GPIIb/IIIa, anti-HLA-DR, and antitransferrin receptor
antibodies. Acid phosphatase staining was also positive. Neither CD34 nor
CD33 antigens were identified on the cells. CD36 and blood group A antigens
were first identified on cells from aggregates containing 32 to 64 cells
after 4 days of secondary culture and preceded the expression of
glycophorin A and hemoglobin alpha. These results indicate that various
cell surface antigens were sequentially expressed during the proliferation
and differentiation of erythroid progenitors, and that our procedure may be
useful for clarifying the morphologic and immunologic properties of
hematopoietic stem cells.
Volume 80,
Issue 3,
pp. 642-650,
08/01/1992
Copyright © 1992 by The American Society of Hematology
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