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Molecular monitoring of the myl/retinoic acid receptor-alpha fusion gene in
acute promyelocytic leukemia by polymerase chain reaction
A Biondi, A Rambaldi, PP Pandolfi, V Rossi, G Giudici, M Alcalay, F Lo Coco, D Diverio, EM Pogliani and EM Lanzi
Clinica Pediatrica Universita di Milano, Ospedale S. Gerardo, Monza, Italy.
The acute promyelocytic leukemia (APL) t(15;17) translocation generates a
myl/retinoic acid receptor-alpha (RAR-alpha) chimeric gene that is
transcribed as a fusion myl/RAR-alpha messenger RNA. Using primer sets
derived from RAR-alpha and myl cDNAs, we were able to amplify the
breakpoint sites of the fusion transcripts of all 35 APL RNA samples by
reverse polymerase chain reaction (PCR) and nested primer approach of two
rounds of amplification. DNA fragments of different size were obtained
according to the chromosome 15 breakpoints (intron 3-bcr 3; exon 6-bcr 2;
and intron 6-bcr 1). bcr 1 and bcr 3 represent the regions of the myl locus
most frequently involved among APL (48.5 and 34.2 of cases, respectively);
bcr 3 constitutes 62.5% of cases among M3V as compared with 25.9% of M3
cases. The feasibility of monitoring the APL clone by PCR analysis in five
APL patients who received different treatment (chemotherapy,
all-trans-retinoic acid or bone marrow transplantation) was evaluated. In
five of nine bone marrow samples of patients in complete remission,
t(15;17)-positive cells could be detected by PCR analysis. We conclude that
PCR amplification of the myl/RAR-alpha junctions represents the easiest and
rapid method for diagnosis and monitoring of the APL clone.
Volume 80,
Issue 2,
pp. 492-497,
07/15/1992
Copyright © 1992 by The American Society of Hematology
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