|
|
 Previous Article | Table of Contents | Next Article 
Human endothelial cells express integrin receptors on the luminal aspect of
their membrane
G Conforti, C Dominguez-Jimenez, A Zanetti, MA Gimbrone , O Cremona, PC Marchisio and E Dejana
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Endothelial cells (EC) form a dynamic interface between blood and the rest
of the body. EC surface properties promoting adhesion of reactive plasma
proteins and/or circulating cells might be of pivotal importance for the
homeostasis of blood and tissues. EC express multiple integrin receptors
that promote their attachment to the subendothelial matrix proteins. Among
these receptors, alpha v beta 3 is of particular relevance on EC, since it
is abundantly expressed and can bind many different matrix and plasma
proteins. It is still unknown whether integrin receptors are selectively
located to the basal side of EC membrane or may also be exposed on the cell
surface in contact with blood. This issue was addressed using different
experimental approaches. First, selective surface radioiodination using
lactoperoxidase (LPO)-latex beads and immunoprecipitation analysis were
performed. We found that cultured EC, similarly to human skin fibroblasts
(HSF), expose alpha v beta 3 on both their apical (free) and basal
(substratum-attached) surfaces. This held also for other integrins such as
alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, and alpha 6 beta 1.
Immunoprecipitation data were verified by morphological techniques.
Immunofluorescence and immunogold-staining of EC with alpha v beta 3, as
well as with beta 1 subfamily antibodies, showed a diffuse and granular
distribution of these integrins on EC surface. alpha v beta 3 and beta 1
integrins were also detected on the apical membrane of EC at higher
magnification by scanning electron microscopy (SEM). Finally, data obtained
on cultured EC were confirmed in vivo on immunogold-labeled ultrathin
cryosections of human vessels by transmission electron microscopy (TEM).
Data indicate, that in addition to their role in promoting EC attachment to
extracellular matrix proteins, integrin receptors of EC can be exposed to
blood-stream and eventually be available for binding of plasma proteins and
circulating cells.
Volume 80,
Issue 2,
pp. 437-446,
07/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Bach-Gansmo, R. Danielsson, A. Saracco, B. Wilczek, T. V. Bogsrud, A. Fangberget, A. Tangerud, and D. Tobin
Integrin Receptor Imaging of Breast Cancer: A Proof-of-Concept Study to Evaluate 99mTc-NC100692
J. Nucl. Med.,
September 1, 2006;
47(9):
1434 - 1439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Urbinati, S. Mitola, E. Tanghetti, C. Kumar, J. Waltenberger, D. Ribatti, M. Presta, and M. Rusnati
Integrin {alpha}V{beta}3 as a Target for Blocking HIV-1 Tat-Induced Endothelial Cell Activation In Vitro and Angiogenesis In Vivo
Arterioscler. Thromb. Vasc. Biol.,
November 1, 2005;
25(11):
2315 - 2320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Urbinati, A. Bugatti, M. Giacca, D. Schlaepfer, M. Presta, and M. Rusnati
{alpha}v{beta}3-integrin-dependent activation of focal adhesion kinase mediates NF-{kappa}B activation and motogenic activity by HIV-1 Tat in endothelial cells
J. Cell Sci.,
September 1, 2005;
118(17):
3949 - 3958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Parsons-Wingerter, I. M. Kasman, S. Norberg, A. Magnussen, S. Zanivan, A. Rissone, P. Baluk, C. J. Favre, U. Jeffry, R. Murray, et al.
Uniform Overexpression and Rapid Accessibility of {alpha}5{beta}1 Integrin on Blood Vessels in Tumors
Am. J. Pathol.,
July 1, 2005;
167(1):
193 - 211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Magnussen, I. M. Kasman, S. Norberg, P. Baluk, R. Murray, and D. M. McDonald
Rapid Access of Antibodies to {alpha}5{beta}1 Integrin Overexpressed on the Luminal Surface of Tumor Blood Vessels
Cancer Res.,
April 1, 2005;
65(7):
2712 - 2721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Sheets, J. Potempa, J. Travis, C. A. Casiano, and H. M. Fletcher
Gingipains from Porphyromonas gingivalis W83 Induce Cell Adhesion Molecule Cleavage and Apoptosis in Endothelial Cells
Infect. Immun.,
March 1, 2005;
73(3):
1543 - 1552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sahni and C. W. Francis
Stimulation of endothelial cell proliferation by FGF-2 in the presence of fibrinogen requires {alpha}v{beta}3
Blood,
December 1, 2004;
104(12):
3635 - 3641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sahni, M. Guo, S. K. Sahni, and C. W. Francis
Interleukin-1{beta} but not IL-1{alpha} binds to fibrinogen and fibrin and has enhanced activity in the bound form
Blood,
July 15, 2004;
104(2):
409 - 414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Holig, M. Bach, T. Volkel, T. Nahde, S. Hoffmann, R. Muller, and R. E. Kontermann
Novel RGD lipopeptides for the targeting of liposomes to integrin-expressing endothelial and melanoma cells
Protein Eng. Des. Sel.,
May 1, 2004;
17(5):
433 - 441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kawasaki, G. E. Davis, and M. J. Davis
Regulation of Ca2+-dependent K+ Current by {alpha}v{beta}3 Integrin Engagement in Vascular Endothelium
J. Biol. Chem.,
March 26, 2004;
279(13):
12959 - 12966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kortesmaa, P. Yurchenco, and K. Tryggvason
Recombinant Laminin-8 (alpha 4beta 1gamma 1). PRODUCTION, PURIFICATION, AND INTERACTIONS WITH INTEGRINS
J. Biol. Chem.,
May 12, 2000;
275(20):
14853 - 14859.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Harlan
Introduction: anti-adhesion therapy in sickle cell disease
Blood,
January 15, 2000;
95(2):
365 - 367.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Singh, C. Fu, and J. Bhattacharya
Vascular expression of the alpha vbeta 3-integrin in lung and other organs
Am J Physiol Lung Cell Mol Physiol,
January 1, 2000;
278(1):
L217 - L226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Wiedle, C. Johnson-Leger, and B. A. Imhof
A Chimeric Cell Adhesion Molecule Mediates Homing of Lymphocytes to Vascularized Tumors
Cancer Res.,
October 1, 1999;
59(20):
5255 - 5263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Chiba, N. Nakagawa, K. Kurasawa, Y. Tanaka, Y. Saito, and I. Iwamoto
Ligation of CD31 (PECAM-1) on Endothelial Cells Increases Adhesive Function of alpha vbeta 3 Integrin and Enhances beta 1 Integrin-Mediated Adhesion of Eosinophils to Endothelial Cells
Blood,
August 15, 1999;
94(4):
1319 - 1329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. V. Byzova and E. F. Plow
Activation of alpha Vbeta 3 on Vascular Cells Controls Recognition of Prothrombin
J. Cell Biol.,
December 28, 1998;
143(7):
2081 - 2092.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. B. Manodori, N. M. Matsui, J. Y. Chen, and S. H. Embury
Enhanced Adherence of Sickle Erythrocytes to Thrombin-Treated Endothelial Cells Involves Interendothelial Cell Gap Formation
Blood,
November 1, 1998;
92(9):
3445 - 3454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. H. Tam, P. M. Sassoli, R. E. Jordan, and M. T. Nakada
Abciximab (ReoPro, Chimeric 7E3 Fab) Demonstrates Equivalent Affinity and Functional Blockade of Glycoprotein IIb/IIIa and {alpha}vß3 Integrins
Circulation,
September 15, 1998;
98(11):
1085 - 1091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Sobel, J. R. Hinojoza, A. Maeda, and M. Chen
Endothelial Cell Integrin Laminin Receptor Expression in Multiple Sclerosis Lesions
Am. J. Pathol.,
August 1, 1998;
153(2):
405 - 415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Heindl, S. Zahler, U. Welsch, and B. F Becker
Disparate effects of adhesion and degranulation of platelets on myocardial and coronary function in postischaemic hearts
Cardiovasc Res,
May 1, 1998;
38(2):
383 - 394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rusnati, E. Tanghetti, P. Dell'Era, A. Gualandris, and M. Presta
alpha vbeta 3 Integrin Mediates the Cell-adhesive Capacity and Biological Activity of Basic Fibroblast Growth Factor (FGF-2) in Cultured Endothelial Cells
Mol. Biol. Cell,
December 1, 1997;
8(12):
2449 - 2461.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. O. Simon, E. M. Nutt, D. G. Abraham, G. A. Rodan, and L. T. Duong
The alpha vbeta 3 Integrin Regulates alpha 5beta 1-mediated Cell Migration toward Fibronectin
J. Biol. Chem.,
November 14, 1997;
272(46):
29380 - 29389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gawaz, F.-J. Neumann, T. Dickfeld, A. Reininger, H. Adelsberger, A. Gebhardt, and A. Schomig
Vitronectin Receptor ({alpha}vß3) Mediates Platelet Adhesion to the Luminal Aspect of Endothelial Cells : Implications for Reperfusion in Acute Myocardial Infarction
Circulation,
September 16, 1997;
96(6):
1809 - 1818.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. A. Beacham, L.-P. Tran, and S. S. Shapiro
Cytokine Treatment of Endothelial Cells Increases Glycoprotein Ibalpha -Dependent Adhesion to von Willebrand Factor
Blood,
June 1, 1997;
89(11):
4071 - 4077.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Styles, B. Lubin, E. Vichinsky, S. Lawrence, M. Hua, S. Test, and F. Kuypers
Decrease of Very Late Activation Antigen-4 and CD36 on Reticulocytes in Sickle Cell Patients Treated With Hydroxyurea
Blood,
April 1, 1997;
89(7):
2554 - 2559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kano, K. Katoh, M. Masuda, and K. Fujiwara
Macromolecular Composition of Stress Fiber–Plasma Membrane Attachment Sites in Endothelial Cells In Situ
Circ. Res.,
November 1, 1996;
79(5):
1000 - 1006.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Tsukada, X. Ying, C. Fu, S. Ishikawa, P. McKeown-Longo, S. Albelda, S. Bhattacharya, B. A. Bray, and J. Bhattacharya
Ligation of Endothelial {alpha}vß3 Integrin Increases Capillary Hydraulic Conductivity of Rat Lung
Circ. Res.,
October 1, 1995;
77(4):
651 - 659.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Bhattacharya, C. Fu, J. Bhattacharya, and S. Greenberg
Soluble Ligands of the [IMAGE][IMAGE][IMAGE][IMAGE] Integrin Mediate Enhanced Tyrosine Phosphorylation of Multiple Proteins in Adherent Bovine Pulmonary Artery Endothelial Cells
J. Biol. Chem.,
July 14, 1995;
270(28):
16781 - 16787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Huebsch, J. B. McCarthy, C. A. Diglio, and D. L. Mooradian
Endothelial Cell Interactions With Synthetic Peptides From the Carboxyl-Terminal Heparin-Binding Domains of Fibronectin
Circ. Res.,
July 1, 1995;
77(1):
43 - 53.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. S. Nicholson, S. G. Panzer-Knodle, A. K. Salyers, B. B. Taite, J. A. Szalony, N. F. Haas, L. W. King, J. A. Zablocki, B. T. Keller, K. Broschat, et al.
SC-54684A: An Orally Active Inhibitor of Platelet Aggregation
Circulation,
January 15, 1995;
91(2):
403 - 410.
[Abstract]
[Full Text]
|
|
|
|
|
