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Erythropoietin-induced stimulation of differentiation and proliferation in
J2E cells is not mimicked by chemical induction
SJ Busfield and SP Klinken
Department of Biochemistry, University of Western Australia, Nedlands.
The J2E cell line is a novel erythroid cell line that differentiates in
response to erythropoietin (Epo), the physiologic stimulus for
erythropoiesis. After exposure to Epo, the cells synthesize hemoglobin, and
we show here that this process is tightly linked to increases in cellular
proliferation and DNA synthesis. The hormone-induced terminal
differentiation also results in morphologic alterations and the
accumulation of transcripts for alpha, beta maj, and beta min globins.
c-myc messenger RNA levels increase rapidly after exposure to Epo and
precede the increase in cell division, while c-myb undergoes a transient
decrease. Differentiation of J2E cells can also be achieved with sodium
butyrate, but, in contrast with Epo, this is associated with a retardation
of replication and a sudden decrease in c-myc levels. These results show
that, in this system, chemically induced differentiation differs from
terminal maturation promoted by Epo and that the processes of proliferation
and differentiation in J2E cells can be uncoupled.
Volume 80,
Issue 2,
pp. 412-419,
07/15/1992
Copyright © 1992 by The American Society of Hematology
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