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Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B'
transcripts during myeloid differentiation of interleukin-3- dependent
murine stem cell lines
IN Hampson, MA Cross, CM Heyworth, L Fairbairn, E Spooncer, GJ Cowling and TM Dexter
Cancer Research Campaign Department of Experimental Hematology, Paterson
Institute for Cancer Research, Withington, Manchester, UK.
Using the technique of differential cDNA library screening, we have
molecularly cloned a gene that is highly expressed in an undifferentiated
myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix)
and that downregulates as these cells are induced to differentiate along
monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of
this gene has shown homology with a previously cloned gene, cytotoxic cell
protease 1 (CCP1 or Granzyme 'B'), that has been shown to be expressed only
in thymocytes, activated T cells, a mast cell line, and peritoneal exudate
leukocytes. In situ hybridization, Northern blot analysis, and nuclear
run-off assay has confirmed that expression of CCP1 is restricted to the
phenotypically primitive multipotent undifferentiated. FDCP-Mix cells that
are undergoing self-renewal in the presence of growth factors such as
interleukin-3.
Volume 80,
Issue 12,
pp. 3097-3105,
12/15/1992
Copyright © 1992 by The American Society of Hematology
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