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T-cell acute lymphoblastic leukemia--the associated gene SCL/tal codes for
a 42-Kd nuclear phosphoprotein
AN Goldfarb, S Goueli, D Mickelson and JM Greenberg
University of Minnesota Hospital Department of Laboratory Medicine and
Pathology, Minneapolis.
SCL/tal is a putative oncogene originally identified through its
involvement in the translocation t(1;14)(p32;q11) present in the leukemic
cell line DU.528. Subsequent studies have shown an upstream deletion
activating expression of SCL/tal to be one of the most common genetic
lesions in T-cell acute lymphoblastic leukemia (T-ALL). The cDNA sequence
of SCL/tal encodes a basic helix-loop-helix (bHLH) protein with regions of
marked homology to lyl-1 and tal-2, two other bHLH proteins involved in
T-ALL chromosomal translocations. The bHLH motif suggests that the SCL/tal
product localizes to the nucleus, binds to specific DNA sequences, and
regulates transcription of a specific array of target genes. Our studies
directly identify the SCL/tal product as a 42-Kd phosphoprotein that
efficiently localizes to the nucleus. Deletion mutagenesis has allowed
identification of a region critical for nuclear localization, a region that
corresponds to the DNA- binding basic domain within the bHLH motif. Because
this domain is shared by lyl-1 and tal-2, these latter putative T-cell
oncoproteins probably use a nuclear localization mechanism identical to
that of SCL/tal.
Volume 80,
Issue 11,
pp. 2858-2866,
12/01/1992
Copyright © 1992 by The American Society of Hematology
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