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Human arterial smooth muscle cells synthesize granulocyte colony-
stimulating factor in response to interleukin-1 alpha and tumor necrosis
factor-alpha
H Zoellner, EL Filonzi, HR Stanton, JE Layton and JA Hamilton
University of Melbourne, Department of Medicine, Royal Melbourne Hospital,
Parkville, Australia.
Vascular smooth muscle cells (SMC) are a major cell type comprising the
walls of blood vessels. We report the synthesis of granulocyte colony-
stimulating factor (G-CSF) by cultured human SMC obtained from the internal
mammary artery and thoracic aorta. Interleukin-1 alpha (IL-1 alpha) greatly
increased in a dose-dependent manner the amount of this cytokine produced
by the SMC, with tumor necrosis factor-alpha (TNF- alpha) being less
effective. Newly formed G-CSF could be detected in culture supernatants
within 6 hours after IL-1 alpha or TNF-alpha treatment. Northern blot
analysis of SMC stimulated with IL-1 alpha and TNF-alpha showed an increase
in the amount of mRNA for G-CSF as compared with control cells. Enhanced
G-CSF mRNA levels were observed when SMC were treated with cycloheximide in
the absence or presence of added cytokine. In vasculitis, the walls of
blood vessels become inflamed as evidenced by a leucocytic infiltrate
usually dominated by polymorphonuclear neutrophil leukocytes (PMNs). G-CSF
is known to stimulate PMNs, and our findings raise the possibility that
G-CSF made by SMC contributes to the development of vasculitis lesions.
Volume 80,
Issue 11,
pp. 2805-2810,
12/01/1992
Copyright © 1992 by The American Society of Hematology
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