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Cell lines produce factors that induce fetal hemoglobin in human BFUe- derived colonies

P Constantoulakis, M Walmsley, R Patient, T Papayannopoulou, T Enver and G Stamatoyannopoulos

Department of Medicine, University of Washington, Seattle 98195.

Established cell lines were screened for secretion of activities than can stimulate fetal hemoglobin (HbF) production in adult burst-forming unit-erythroid (BFUe) cultures. Conditioned media from four cell lines, a human teratocarcinoma, an osteosarcoma, a bladder cell carcinoma, and feline leukemia virus (FeLV) A-infected feline fibroblasts (FEF-A cells), consistently increased the relative production of fetal globin in BFUe-derived colonies. In vitro translation of RNA from these cells in Xenopus oocytes yielded products that increased the gamma to gamma+beta ratio in adult erythroid colonies. These results demonstrate that a variety of cell lines produce factors that stimulate the production of HbF in vitro. The genes of such factors could be isolated by expression cloning of cDNA from cell lines using the Xenopus oocyte system.

Volume 80, Issue 10, pp. 2650-2655, 11/15/1992
Copyright © 1992 by The American Society of Hematology


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  Copyright © 1992 by American Society of Hematology         Online ISSN: 1528-0020