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Separation of hematopoietic stem cells into two populations and their
characterization
H Ogata, S Taniguchi, M Inaba, M Sugawara, Y Ohta, K Inaba, KJ Mori and S Ikehara
First Department of Pathology, Kansai Medical University, Osaka, Japan.
Two populations of hematopoietic stem cells (HSC) in mouse bone marrow (BM)
are defined on the basis of the presence or absence of interleukin- 3
(IL-3) receptor-associated antigen (IL-3RAA). HSC were purified by
depletion of mature lymphoid-lineage cells followed by collection of the
low-density fraction and sorting of wheat germ agglutinin-binding (WGA+)
cells using a fluorescein-activated cell sorter. WGA+ cells were further
separated into two populations (IL-3RAA+/WGA+ and IL-3RAA- /WGA+) by a
monoclonal antibody (MoAb) against IL-3RAA. IL-3RAA+/WGA+ cells formed
CFU-S on day 8; this population consisted mainly of cells in the cycling
phase. IL-3RAA-/WGA+ cells form CFU-S on day 12; this population consisted
mainly of dormant cells (cells in the G0 phase). When two populations
obtained from C3H/HeN mice were injected into lethally irradiated (C57BL/6
x C3H/HeN)F1 mice, donor-derived cells in the peripheral blood (PB)
appeared significantly earlier in mice injected with IL-3RAA+/WGA+ cells
than in those injected with IL-3RAA- /WGA+ cells, whereas the
reconstruction efficiency of IL-3RAA-/WGA+ cells had overtaken that of
IL-3RAA+/WGA+ cells 6 weeks after injection. Long-term observation showed
no significant difference between these two populations, however. The
radioprotective ability (RPA) (30-day survival) of these two populations
was therefore compared. The RPA of IL-3RAA-/WGA+ cells was significantly
higher than that of IL-3RAA+/WGA+ cells. These findings therefore suggest
that the former population is more primitive.
Volume 80,
Issue 1,
pp. 91-95,
07/01/1992
Copyright © 1992 by The American Society of Hematology
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