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Down-regulation of human protein kinase C alpha is associated with terminal
neutrophil differentiation
V Devalia, NS Thomas, PJ Roberts, HM Jones and DC Linch
Department of Haematology, University College and Middlesex School of
Medicine, London, England.
We have established an RNase protection method to quantify the expression
of mRNA for the human protein kinase C (PK-C) isoforms alpha, beta 1, beta
2, and gamma. This was used to investigate whether each isoform is
differentially expressed during the differentiation of hematopoietic cells.
Myeloid and lymphoid cells express PK-C alpha, beta 1, and beta 2 mRNAs in
various proportions. PK-C gamma mRNA was detected in human brain, but not
in hematopoietic cells. PK-C alpha mRNA decreases as HL-60 cells mature to
a neutrophil phenotype in response to retinoic acid, but its abundance does
not change during monocytic differentiation in response to vitamin D3. PK-C
alpha mRNA and protein were undetectable in peripheral blood neutrophils,
but are present in monocytes. The mRNAs for PK-C beta 1 and beta 2 isoforms
increase during HL-60 differentiation and are expressed in both neutrophils
and monocytes. Therefore, the PK-C alpha isoform is specifically
down-regulated during human neutrophil terminal differentiation. These data
suggest that mature neutrophil functions do not require the PK-C alpha
isoform.
Volume 80,
Issue 1,
pp. 68-76,
07/01/1992
Copyright © 1992 by The American Society of Hematology
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