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Characterization, growth, and differentiation of a human myeloid leukemia
cell line, TI-1 cell
T Taoka, T Tasaka, T Tanaka, S Irino and AW Norman
Division of Biomedical Sciences, University of California, Riverside 92521.
A cell line (TI-1) has been established from the peripheral blood of a
patient with acute myeloid leukemia (M2). A typical TI-1 cell displayed
many abnormalities of its chromosomes, but not the Philadelphia (Ph1)
chromosome. Light and electron microscopic examination and histochemical
analysis indicated that the TI-1 cells were undifferentiated blast cells,
but immunologic marker studies suggested that these cells had myeloid
characteristics. The proliferation of TI-1 cells was dependent on the
concentration of fetal bovine serum (FBS). Their doubling time was 13.8
hours when they were cultured in a medium containing 10% FBS. Phorbol-12
myristate 13-acetate (PMA) induced the TI-1 cells to differentiate into
monocyte-like cells, as judged by their morphologic similarity to
monocytes, their adhesion to the culture dish, and their increase of both
nitroblue tetrazolium (NBT)- reducing ability and nonspecific esterase
(NSE)-activity. PMA significantly inhibited the proliferation and DNA
synthesis of TI-1 cells in a dose-dependent manner. The PMA-induced
differentiation was significantly inhibited by the protein kinase C
inhibitors (H-7, staurosporine). Hemin induced the TI-1 cells to
differentiate into erythroid cells. The number of hemoglobin-producing
cells and hemoglobin production was increased by hemin treatment. Hemin
also inhibited the proliferation of the TI-1 cells. Thus, the TI-1 cell
represents a bipotent, granulo-monocytoid, and erythroid cell line. The
TI-1 cell line will be a useful model for monocytoid and erythroid
differentiation.
Volume 80,
Issue 1,
pp. 46-52,
07/01/1992
Copyright © 1992 by The American Society of Hematology
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