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Effects of gamma-interferon on human neutrophils: protection from
deterioration on storage
SJ Klebanoff, S Olszowski, WC Van Voorhis, JA Ledbetter, AM Waltersdorph and KG Schlechte
Department of Medicine, University of Washington, Seattle 98195.
Human polymorphonuclear leukocytes (PMN) preincubated overnight with 100
U/mL gamma-interferon (IFN-gamma) had an increased metabolic response, as
measured by iodination and/or superoxide production, to stimulation by
tumor necrosis factor (TNF), granulocyte-macrophage colony-stimulating
factor (GM-CSF), formylmethionyl-leucyl- phenylalanine (FMLP), opsonized
zymosan, and lipopolysaccharide (LPS), as compared with cells comparably
preincubated in the absence of IFN- gamma. The decline in the staphylocidal
activity of the stored PMN was also prevented in part by IFN-gamma, as was
the depressed adherence of PMN stimulated with phorbol myristate acetate
(PMA), FMLP, TNF, GM-CSF, and LPS. This protective effect of IFN-gamma on
PMN function was associated with the prolonged surface expression of the
complement receptor three (CR3) alpha-chain (CD11b), CR3 beta-chain (CD18),
FcRII (CD32), and FcRIII (CD16), and the appearance of surface FcRI (CD64).
The polymerase chain reaction (PCR) was used to amplify neutrophil RNA-
derived cDNA recognized by synthetic oliogonucleotides designed from
published nucleotide sequences for specific proteins. Using this procedure,
mRNA for gp91-phox, p67-phox, p47-phox, CD64, two forms of CD32, CD16,
CD11b, CD18, and actin were found to be depressed after overnight storage
of neutrophils, and this decrease in steady-state mRNA levels was in part
or totally prevented by IFN-gamma. CD64 and gp91-phox mRNA were generally
increased by IFN-gamma to a level greater than that of freshly isolated
neutrophils. Northern analysis of CD64 and p47 phox mRNAs confirmed the
findings with the PCR method. These findings suggest that storage of PMN in
a functionally active state is favored by the presence of IFN-gamma.
Volume 80,
Issue 1,
pp. 225-234,
07/01/1992
Copyright © 1992 by The American Society of Hematology
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