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Clonal analysis of myelodysplastic syndrome: monosomy 7 is expressed in the
myeloid lineage, but not in the lymphoid lineage as detected by fluorescent
in situ hybridization
WR Gerritsen, J Donohue, J Bauman, SC Jhanwar, NA Kernan, H Castro-Malaspina, RJ O'Reilly and JH Bourhis
Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York,
NY 10021.
Conflicting results have been published on whether or not myelodysplastic
syndromes (MDS) affect all cell lineages. Involvement of myeloid and
erythroid cell lineages has been regularly observed, but it remains
controversial whether the different lymphoid cell lineages are involved. In
this study of eight patients with MDS associated with monosomy 7,
fluorescent in situ hybridization (FISH) was used to enumerate the
chromosomes 7 in interphase cells. With the probe D7Z1, the rate of
false-positive detection of monosomy 7 was 3% +/- 2% in normal cells. T-
and B-cell lines were established from eight patients with MDS and monosomy
7. As determined by FISH in interphase cells, 1.9% (0% to 3%) of the cells
in the B-cell lines showed one fluorescent spot and 1.1% (0% to 2.9%) of
the cells in the T-cell lines. These values do not differ from normal
values. However, the possibility that normal cells were selected when the
T- and B-cell lines were established could not be excluded. Therefore,
peripheral blood cells were obtained, separated according to surface
markers specific for lymphoid and myeloid cell lineage with a cell sorter,
and analyzed for the expression of monosomy 7 by FISH. Antibodies
recognizing T cells (CD3), B cells (CD20), natural killer (NK) cells
(CD57), monocytes and granulocytes (low and high expression of CD11b
antigen), and myeloid progenitors (CD33) were used to separate cells. The
expression of monosomy 7 in the T cells, NK cells, and B cells did not
differ from control values. These results in the lymphoid subpopulations
are in stark contrast with the observations in the myeloid populations; the
percentage of cells with monosomy 7 ranged from 9% to 78% (controls: 6% +/-
2%) in cells with low CD11b expression, 20% to 89% in cells with a high
expression of the CD11b antigen (controls: 7% +/- 3%), and 23% to 91% in
the CD33 positive cells (controls: 5% +/- 3%). The results of this study
suggest that monosomy 7 does not usually affect lymphoid subpopulations but
is restricted to committed progenitor cells with the capacity to
differentiate into mature myeloid cells.
Volume 80,
Issue 1,
pp. 217-224,
07/01/1992
Copyright © 1992 by The American Society of Hematology
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