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A novel human lymphoma cell line (Deglis) with dual B/T phenotype and gene
rearrangements and containing Epstein-Barr virus genomes
T al Saati, HJ Delecluze, S Chittal, P Brousset, JP Magaud, N Dastugue, E Cohen- Knafo, G Laurent, B Rubin and G Delsol
Groupe d'Etude des Lymphomes Malins, CHU Purpan, Toulouse, France.
We report a new and apparently unique human lymphoma cell line termed
Deglis. The line was established from a polymorphic centroblastic lymphoma.
The cell line and its source carry a dual B-cell and T-cell phenotype and
Epstein-Barr virus (EBV) genomes. Simultaneous expression of B-cell (CD19+,
CD20+, CD23+, CD37+) and T-cell (CD2+, CD3+/-, CD7+, CD43+) antigens,
activation antigens (CD30+, CDw70+) as well as CD68+, a
macrophage-associated antigen, was observed on the cell line and its
source. Genotypic studies of the cell line showed dual gene rearrangements.
JH (on both primary tumor and the cell line) and C kappa were rearranged
without expression of cytoplasmic or surface immunoglobulins. T-cell
receptor-alpha (TCR-alpha) and TCR-beta genes were rearranged, but
TCR-delta and TCR-gamma genes were in germline configuration. Apparently,
functional transcripts of TCR-alpha and truncated transcripts for TCR-beta
and TCR-delta were observed. EBV- encoded proteins (LMP and EBNA2) were
expressed by the parent tumor and the cell line. Southern blot analysis
showed the same clonal EBV genomes in the primary tumor and the cell line.
Karyotypic analysis of the cell line showed several chromosomal
abnormalities but normal chromosomal number. The characteristics of this
cell line suggest that neoplastic transformation has occurred in a
precursor cell broadly committed to lymphoid lineage. Further studies on
this cell line may help resolve some issues in the physiopathology of
lymphoid tumors.
Volume 80,
Issue 1,
pp. 209-216,
07/01/1992
Copyright © 1992 by The American Society of Hematology
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