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Infection of hematopoietic progenitor cells by human cytomegalovirus
JP Maciejewski, EE Bruening, RE Donahue, ES Mocarski, NS Young and SC St Jeor
National Heart, Lung and Blood Institute, Cell Biology Section, National
Institutes of Health, Bethesda, MD.
The susceptibility of hematopoietic progenitor cells to infection by human
cytomegalovirus (HCMV) was investigated using several strains of HCMV,
including the recombinant strain RC256. RC256 is derived from the
laboratory strain Towne and contains the Escherichia coli LacZ gene coding
for beta-galactosidase (beta-gal) regulated by an early HCMV promoter.
Expression of LacZ allowed the detection of HCMV in individual
hematopoietic cells. Clonogeneic bone marrow (BM) progenitors, including
CD34+ cells, could be infected with HCMV and would then form normal
hematopoietic colonies. By polymerase chain reaction (PCR) amplification of
DNA, HCMV could be detected in both erythroid and myeloid colonies. LacZ
activity was observed predominantly in cells of myelomonocytic lineage.
When cells derived from HCMV-infected progenitors were cocultivated with
permissive human fibroblasts, infectious virus expressing LacZ was
recovered. Although no characteristic HCMV cytopathology was observed in BM
colonies, high virus to cell ratios resulted in a moderate inhibition of
colony formation. Since infected hematopoietic progenitors can harbor HCMV
for weeks and through several differentiation steps in culture, we
postulate that in vivo these cells may serve as a reservoir of latent virus
and contribute to HCMV dissemination.
Volume 80,
Issue 1,
pp. 170-178,
07/01/1992
Copyright © 1992 by The American Society of Hematology
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