Blood, 1953, Vol. 8, No. 5, pp. 399-412.
© 1953 American Society of Hematology, Inc.
Immunologic Studies of Hemoglobins
I. The Production of Antihemoglobin Sera and Their
Immunologic Characteristics
AMOZ I. CHERNOFF M.D.1
1 Department of Medicine, Washington University School of Medicine, St.
Louis, Mo.
1. Antisera to hemoglobin solutions obtained from normal adult, cord blood
and sickle cell anemia erythrocytes have been prepared by immunizing rabbits
with adjuvant mixtures. Furthermore, anti F hemoglobin sera have been similarly prepared by using purified fetal hemoglobin. A species specific anti guinea
pig hemoglobin serum was also developed.
2. Although anti F hemoglobin sera appear to be specific for fetal hemoglobin,
no such immunologic specificity could be detected between anti A and anti
SCA sera obtained from rabbits.
3. It is suggested that sera prepared by using hemoglobin solutions obtained
from normal and sickle cell anemia erythrocytes may contain anti F antibodies in
addition to anti A and anti S precipitins and that fetal hemoglobin may be present in many human adult blood specimens.
Submitted on November 19, 1952
Accepted on January 31, 1953
