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1 Laboratory of Experimental Oncology, National Cancer Institute, National
Institutes of Health, Public Health Service, Federal Security Agency and the Department
of Medicine and Cancer Research Institute, University of California School of Medicine,
San Francisco, Calif.
The DNA content, in arbitrary units, of individual circulating lymphocytes
from nine normal subjects, nine patients with chronic lymphocytic leukemia, and
five patients with acute lymphocytic leukemia was estimated microspectrophotometrically with the Feulgen dye. Normal circulating lymphocytes were
found to contain twice the average DNA content of normal human spermatids,
corroborating their diploid chromosome number. Lymphocytes from normal and
leukemic lymph node and bone marrow were frequently found possessing four
times the average spermatid DNA value. Three out of nine patients with chronic
lymphocytic leukemia, and four of five patients with acute lymphocytic leukemia
had significantly increased numbers of circulating lymphocytes containing DNA
values which were elevated above the normal diploid value. The patients demonstrating cells with elevated DNA values were in a clinically exacerbated phase of
their disease. The significance of these findings is discussed.
This article has been cited by other articles:
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| Copyright © 1953 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
