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Direct detection of activated protein C in blood from human subjects
A Gruber and JH Griffin
Department of Molecular and Experimental Medicine, Scripps Research
Institute, La Jolla, CA 92037.
The antithrombotic enzyme, activated protein C (APC) was measured in blood
using an enzyme capture assay (ECA). The ECA involved (1) collection of
blood into anticoagulant containing a reversible inhibitor of the enzyme,
(2) specific affinity capture of the enzyme by an immobilized antibody that
does not inhibit the enzyme, (3) removal of the reversible inhibitor by
washing, and (4) direct assay of the captured enzyme's amidolytic activity.
The ECA for APC used benzamidine for inhibition, anti-PC light-chain murine
monoclonal antibody for capture, and the oligopeptide substrate S-2366 for
enzyme assay. The sensitivity of this assay was 5 pmol/L (0.3 ng/mL) APC.
The APC activity in normal pooled plasma corresponded to the amidolytic
activity of 38 pmol/L (2.26 +/- 0.2 ng/mL) purified human plasma- derived
APC in the ECA. APC levels in 41 normal donors ranged from 64% to 143%,
averaging 104.9% +/- 19.6% (SD). Thus, APC is a measurable and normal
component of circulating human blood, and this ECA may be useful for
identifying APC deficiency. Moreover, similar ECAs for other enzymes in the
circulation may be useful.
Volume 79,
Issue 9,
pp. 2340-2348,
05/01/1992
Copyright © 1992 by The American Society of Hematology
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A. R. Rezaie
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