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Isolation and molecular characterization of the human CD34 gene
XY He, VP Antao, D Basila, JC Marx and BR Davis
Geraldine Brush Cancer Research Institute, Medical Research Institute of
San Francisco, CA 94115.
The human CD34 surface antigen is selectively expressed on hematopoietic
stem/progenitor cells, suggesting that it plays an essential role in early
hematopoiesis. Using a 1.5-kb partial human CD34 cDNA sequence,
RNA-polymerase chain reaction (PCR), and rapid amplification of cDNA ends
(RACE) methods, we cloned and sequenced the full-length (2.65 kb) cDNA. The
cDNA encodes a type I transmembrane protein with no obvious homology to
other known proteins. The entire CD34 gene of 28 kb was cloned, and the
coding sequences mapped to eight exons. Mapping of the 5' termini of mRNAs
by 5'-RACE and RNAase protection analyses has indicated that the human CD34
gene uses multiple transcription initiation sites. Analysis of the upstream
regulatory sequences revealed the absence of TATA and CAAT box sequences,
and the presence of myb, myc, and ets-like DNA binding motifs. We have
identified significant homology between human and mouse CD34 genes in 5'
and 3' untranslated regions, amino acid coding sequences, and 5' flanking
sequences. This investigation of the CD34 gene should facilitate study of
the function and regulation of this stem cell antigen.
Volume 79,
Issue 9,
pp. 2296-2302,
05/01/1992
Copyright © 1992 by The American Society of Hematology
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