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S Kojima, T Matsuyama and Y Kodera
Division of Hematology and Oncology, Children's Medical Center, Japanese
Red Cross Nagoya First Hospital.
We studied the production of granulocyte colony-stimulating factor (G-
CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and
interleukin-6 (IL-6) by stromal cells from 33 patients with aplastic anemia
(AA). Complete, confluent stromal layers were produced by 29 of the 33
samples using the long-term bone marrow culture (LTBMC) system. The
concentration of G-CSF, GM-CSF, and IL-6 in culture media with or without
interleukin-1 (IL-1) stimulation was determined by an enzyme- linked
immunoadsorbent assay (ELISA). The spontaneous production of G- CSF,
GM-CSF, and IL-6 did not differ significantly between normal controls and
the patients with AA. The ability of stromal cells to release the three
hematopoietic growth factors in response to IL-1 was either normal or
elevated in all but one patient. We also studied the change in production
of G-CSF, GM-CSF, and IL-6 by stromal cells before and after antilymphocyte
globulin (ALG) therapy in 16 patients with AA. There was no correlation
between the change in production of these cytokines and the response to
ALG. In contrast to previous studies that showed a defect in the production
of hematopoietic growth factors by stromal cells from patients with AA, the
results indicated a normal or elevated production of G-CSF, GM-CSF, and
IL-6 by marrow stromal cells in patients with AA.
This article has been cited by other articles:
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| Copyright © 1992 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
