|
|
 Previous Article | Table of Contents | Next Article 
Inhibition of c-jun causes reversible proliferative arrest and withdrawal
from the cell cycle
MJ Smith and EV Prochownik
Division of Hematology/Oncology, University of Michigan School of Medicine,
Ann Arbor 48109.
We studied the effect of c-jun depletion in Friend murine erythroleukemia
(F-MEL) cells stably transfected with a plasmid that allowed for the
glucocorticoid-mediated conditional expression of c-jun antisense
sequences. The c-jun cDNA used for the construction of the vector was
modified so as to prevent the nonspecific targeting of junB and junD
transcripts. High level and rapid induction of c-jun antisense transcripts
was achieved with as little as 10(-8) mol/L dexamethasone (DEX) and
resulted in a 80% to 90% reduction in c-jun protein levels. The continuous
exposure of the transfected cells to DEX inhibited growth by greater than
85% over a 5-day period, whereas DEX had no effect on the growth rate of
control F-MEL cells. This proliferative block was associated with a
reversible accumulation of cells with a 2n DNA content. When these cells
were recultured in the absence of DEX, c- jun protein rapidly reappeared
and the immediate early response genes egr-1, junB, and c-myc were
transiently expressed. Thus, inhibition of c-jun protein causes
logarithmically growing cells to leave the cell cycle and to enter a state
closely resembling, if not identical to, G0. These results underscore the
importance of c-jun in maintaining cellular proliferation and provide
additional evidence for the participation of proto-oncogenes in cell cycle
control.
Volume 79,
Issue 8,
pp. 2107-2115,
04/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. R. Himes, D. P. Sester, T. Ravasi, S. L. Cronau, T. Sasmono, and D. A. Hume
The JNK Are Important for Development and Survival of Macrophages
J. Immunol.,
February 15, 2006;
176(4):
2219 - 2228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Chu, J. Guo, and C.-Y. Chen
Long-term Exposure to Nicotine, via Ras Pathway, Induces Cyclin D1 to Stimulate G1 Cell Cycle Transition
J. Biol. Chem.,
February 25, 2005;
280(8):
6369 - 6379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Jacobs-Helber and S. T. Sawyer
Jun N-terminal kinase promotes proliferation of immature erythroid cells and erythropoietin-dependent cell lines
Blood,
August 1, 2004;
104(3):
696 - 703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chung, C. Liu, D. E. Smith, H. K. Seitz, R. M. Russell, and X.-D. Wang
Restoration of retinoic acid concentration supresses ethanol-enhanced c-Jun expression and hepatocyte proliferation in rat liver
Carcinogenesis,
August 1, 2001;
22(8):
1213 - 1219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Clark, E. J. Black, A. MacLaren, U. Kruse, N. LaThangue, P. K. Vogt, and D. A. F. Gillespie
v-Jun Overrides the Mitogen Dependence of S-Phase Entry by Deregulating Retinoblastoma Protein Phosphorylation and E2F-Pocket Protein Interactions as a Consequence of Enhanced Cyclin E-cdk2 Catalytic Activity
Mol. Cell. Biol.,
April 1, 2000;
20(7):
2529 - 2542.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Kondo, T. Matsuda, T. Kitano, A. Takahashi, M. Tashima, H. Ishikura, H. Umehara, N. Domae, T. Uchiyama, and T. Okazaki
Role of c-jun Expression Increased by Heat Shock- and Ceramide-activated Caspase-3 in HL-60 Cell Apoptosis. POSSIBLE INVOLVEMENT OF CERAMIDE IN HEAT SHOCK-INDUCED APOPTOSIS
J. Biol. Chem.,
March 10, 2000;
275(11):
7668 - 7676.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schreiber, A. Kolbus, F. Piu, A. Szabowski, U. Möhle-Steinlein, J. Tian, M. Karin, P. Angel, and E. F. Wagner
Control of cell cycle progression by c-Jun is p53 dependent
Genes & Dev.,
March 1, 1999;
13(5):
607 - 619.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. M. Stepan, M. Tatewaki, M. Matsushima, C. J. Dickinson, J. del Valle, and A. Todisco
Gastrin induces c-fos gene transcription via multiple signaling pathways
Am J Physiol Gastrointest Liver Physiol,
February 1, 1999;
276(2):
G415 - G424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Patel and J.-Y. Wang
Polyamine depletion is associated with an increase in JunD/AP-1 activity in small intestinal crypt cells
Am J Physiol Gastrointest Liver Physiol,
February 1, 1999;
276(2):
G441 - G450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Wei, N. Inamdar, and W. V. Vedeckis
Transrepression of c-jun Gene Expression by the Glucocorticoid Receptor Requires Both AP-1 Sites in the c-jun Promoter
Mol. Endocrinol.,
September 1, 1998;
12(9):
1322 - 1333.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A.-K. Yi, M. Chang, D. W. Peckham, A. M. Krieg, and R. F. Ashman
CpG Oligodeoxyribonucleotides Rescue Mature Spleen B Cells from Spontaneous Apoptosis and Promote Cell Cycle Entry
J. Immunol.,
June 15, 1998;
160(12):
5898 - 5906.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. F. Hales
Oxidative Stress Regulates the Expression and Activity of Transcription Factor Activator Protein-1 in Rat Conceptus
J. Pharmacol. Exp. Ther.,
February 1, 1997;
280(2):
1085 - 1093.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Todisco, Y. Takeuchi, C. Seva, C. J. Dickinson, and T. Yamada
Gastrin and Glycine-extended Progastrin Processing Intermediates Induce Different Programs of Early Gene Activation
J. Biol. Chem.,
November 24, 1995;
270(47):
28337 - 28341.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Sawai, T. Okazaki, H. Yamamoto, H. Okano, Y. Takeda, M. Tashima, H. Sawada, M. Okuma, H. Ishikura, H. Umehara, et al.
Requirement of AP-1 for Ceramide-induced Apoptosis in Human Leukemia HL-60 Cells
J. Biol. Chem.,
November 10, 1995;
270(45):
27326 - 27331.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Albanese, J. Johnson, G. Watanabe, N. Eklund, D. Vu, A. Arnold, and R. G. Pestell
Transforming p21[IMAGE] Mutants and c-Ets-2 Activate the Cyclin D1 Promoter through Distinguishable Regions
J. Biol. Chem.,
October 6, 1995;
270(40):
23589 - 23597.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Pestell, C. Albanese, G. Watanabe, J. Johnson, N. Eklund, P. Lastowiecki, and J. L. Jameson
Epidermal Growth Factor and c-Jun Act via a Common DNA Regulatory Element to Stimulate Transcription of the Ovine P-450 Cholesterol Side Chain Cleavage (CYP11A1) Promoter
J. Biol. Chem.,
August 4, 1995;
270(31):
18301 - 18308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
