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Integrin expression profiles during erythroid differentiation
T Papayannopoulou and M Brice
Department of Medicine, University of Washington, Seattle 98195.
To study the expression of integrins at the erythroid progenitor level we
isolated selected populations of cells from human fetal liver after
immunoadherence to anti-beta 2 integrin (CD18) coated plates. These CD18
adherent cells (CD18-Ad), in contrast to CD18 nonadherent cells (CD18-NAd),
have a blastlike cell morphology and are highly enriched in all progenitor
types (14% to 37% progenitors). By several criteria progenitor cells
present in CD18-Ad cells appear to have a higher proliferative potential
and diversity than the ones found in CD18-NAd, which were mostly later
erythroid progenitors. Positivity of CD18-Ad cells with the common beta 2
integrin (CD18) is largely attributable to expression of alpha L (CD11a)
chain, rather than alpha M (CD11b). CD11a is present in all types of
progenitors, but it is selectively lost at later stages of erythroid
differentiation/maturation. By contrast, CD11b appears to be virtually
absent from all progenitors but it has an enhanced expression during
granulomonocytic differentiation/maturation. In addition to beta 2
integrins, CD18-Ad cells express several other cytoadhesion molecules
(VLA-4, VLA-5, I-CAM, H-CAM) as well as other progenitor cell antigens
(CD34, HLA-DR, CD38). Cells expressing all these antigens were selectively
enriched in CD18-Ad cells. Our data add new information on the regulation
of CD11a and CD11b molecules in hematopoiesis and on the composite profile
of integrin expression at several stages of erythroid differentiation.
Volume 79,
Issue 7,
pp. 1686-1694,
04/01/1992
Copyright © 1992 by The American Society of Hematology
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