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KA Bobolis, JJ Moulds and MJ Telen
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
JMH is a high-frequency human erythrocyte blood group antigen. Previous
work has shown that JMH is absent from complement-sensitive erythrocytes of
patients with paroxysmal nocturnal hemoglobinuria (PNH); such cells have a
broad defect in expression of phosphatidylinositol (PI)-linked proteins.
Using both human JMH antisera and a JMH-like murine monoclonal antibody, we
have identified a 76-Kd membrane protein present in JMH-positive but not
JMH-negative erythrocytes. A similar 76-Kd JMH protein was also identified
on a human lymphoid T-cell line, HSB-2. Using PI-specific phospholipase C,
a small amount of JMH antigen could be cleaved from intact erythrocytes and
immunoprecipitated from the supernate of treated erythrocytes, thus
confirming that the protein bearing the JMH antigen is anchored by a PI-
linkage to the erythrocyte membrane. This protein was further shown not to
be identical to decay accelerating factor (70 Kd), a previously identified
PI-anchored protein of somewhat similar molecular weight.
This article has been cited by other articles:
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| Copyright © 1992 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
