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Rapid priming of human monocytes by human hematopoietic growth factors:
granulocyte-macrophage colony-stimulating factor (CSF), macrophage-CSF, and
interleukin-3 selectively enhance superoxide release triggered by
receptor-mediated agonists
A Yuo, S Kitagawa, K Motoyoshi, E Azuma, M Saito and F Takaku
Clinical Research Institute, National Medical Center, Tokyo, Japan.
The effects of hematopoietic growth factors on human monocyte superoxide
(O2-) release were investigated by using purified human monocytes in
suspension. Among growth factors studied, granulocyte- macrophage
colony-stimulating factor (GM-CSF), macrophage-CSF (M-CSF), and
interleukin-3 (IL-3) primed human monocytes and enhanced O2- release
stimulated by the receptor-mediated agonists, N-formyl-
methionyl-leucyl-phenylalanine (FMLP) and concanavalin A (Con A), but not
by phorbol myristate acetate, which bypasses the receptors to stimulate the
cells. The optimal priming was obtained by pretreatment of cells with 1 to
5 ng/mL (0.07 to 0.34 nmol/L) GM-CSF, 50 to 100 ng/mL (0.5 to 1.1 nmol/L)
M-CSF, or 10 to 20 ng/mL (0.6 to 1.3 nmol/L) IL-3 for 10 minutes at 37
degrees C. Potency of the maximal priming effects on FMLP- or Con A-induced
O2- release was GM-CSF greater than M- CSF = IL-3. The combination of the
optimal concentrations of any two CSFs resulted in the effect of more
potent priming agent alone. Enhancement of O2- release by GM-CSF was
observed over the complete range of effective concentrations of FMLP
(10(-8) to 10(-6) mol/L). The pretreatment of monocytes with
granulocyte-CSF (50 ng/mL), interferon- gamma (1,000 U/mL), or IL-4 (20
ng/mL) for 10 minutes at 37 degrees C had no effect on O2- release
stimulated by FMLP or Con A. These findings show that GM-CSF, M-CSF, and
IL-3 selectively enhance O2- release in human monocytes triggered by
receptor-mediated agonists after short-term preincubation.
Volume 79,
Issue 6,
pp. 1553-1557,
03/15/1992
Copyright © 1992 by The American Society of Hematology
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