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Heme oxygenase is a positive acute-phase reactant in human Hep3B hepatoma
cells
K Mitani, H Fujita, A Kappas and S Sassa
Laboratory of Metabolism and Pharmacology, Rockefeller University Hospital,
New York, NY 10021.
The effects of human interleukin-6 (hIL-6), the major acute-phase inducer,
on the level of the transcript of microsomal heme oxygenase (HO) were
examined in a human hepatoma cell line, Hep3B. Messenger RNAs (mRNAs)
encoding HO and haptoglobin (Hpt) increased after hIL-6 treatment in a
time- and dose-dependent manner. hIL-6 had no effect on the induction of
heat-shock protein 70 (hsp70) mRNA, suggesting that the induction of HO by
hIL-6 is regulated by a different mechanism from that which mediates the
heat-shock induction of this enzyme. The hIL-6- mediated induction of HO
mRNA was completely abrogated by simultaneous treatment of cells with
actinomycin D, but not with cycloheximide, suggesting that the induction
occurs at the level of transcription. A nuclear factor was shown both in
untreated, and in the hIL-6-treated Hep3B cells that binds specifically to
the IL-6-responsive element (IL6- RE) of the human HO gene. These findings
suggest that HO is a positive acute-phase reactant in this human
liver-derived cell line, and that the nuclear factor specific to the IL6-RE
may be involved in the activation of the HO gene after hIL-6 treatment.
Volume 79,
Issue 5,
pp. 1255-1259,
03/01/1992
Copyright © 1992 by The American Society of Hematology
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